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Molecular Bacteriology pp 387–406Cite as

Diagnosisand Investigation of Diarrheagenic Escherichia coli

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 15))

Abstract

Although most Escherichia coli are harmless commensals of the human intestine, certain specific, highly-adapted E. coli strains are capable of causing urinary tract, systemic or enteric/diarrheagenic infection. Diarrheagenic E coli are divided into six distinct categories, or pathotypes, each with a distinct pathogenic scheme (Table 1). Combined, diarrheagenic E coli have emerged as perhaps the most important enteric pathogens of man. In the developing world, the E coli categories account for more cases of gastroenteiltis among infants than any other cause (1) In addition, E coli are also the most common cause of traveller’s diarrhea, which afflicts more than one million travellers to the developing world annually (1). Enterohemorrhagic E coli (EHEC) are the cause of hemolytic uremic syndrome (HUS), which has become a major foodborne threat in many parts of the developed world (2).

Table 1 Categories of Diarrheagenic E. coli
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References

  1. Levine, M.M (1987) Escherichia coli that cause diarrhea enterotoxigenic,enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent J Infect Dis 155, 377–389

    PubMed  CAS  Google Scholar 

  2. Pickering, L K., Obng, T G., and Stapleton, F. B (1994) Hemolytic-uremicsyndrome and enterohemorrhagic Escherichia coli Pediatr Infect Dis J 13, 459–476

    CAS  Google Scholar 

  3. Albert, M. J, Faruque, S M, Faruque, A S. G, Neogi, P. K. B, Ansaruzzaman, M, Bhuiyan, N A, Alam, K, and Akbar, M S (1995) Controlled study ofEscherichza colidiarrhea1 mfectlons in Bangladesh1 children J Clin Microbiol 33, 973–977

    PubMed  CAS  Google Scholar 

  4. Levine, M. M (1985) Escherzchza coli lmfections N Engl J Med 313, 445–447

    Article  PubMed  CAS  Google Scholar 

  5. Echevema, P., Taylor, D N., Donohue-Rolfe, A., Supawat, K., Ratchtrachenchal, O, Kaper, J., and Keusch, G T.(1987) HeLa cell adherence and cytotoxm production by enteropathogemc Escherichia coli isolated from Infants with diarrheain Thailand J Clzn Mzcrobzol 25, 1519–1523

    Google Scholar 

  6. Cassels, F J and Wolf, M K (1995) Colomzatlon factors of dlarrheagemc E coliand their mtestmal receptors J Indust Microbiol 15, 214–226.

    Article  CAS  Google Scholar 

  7. Moseley, S L, Echevema, P., Serwatana, J, Tirapat, C, Chalcumpa, W, Sakuldaipeara, T., and Falkow, S (1982) Identlfication of enterotoxlgenicEscherichia co1i by colony hybridization using three enterotoxin gene probes JInfect Dis 145, 863–869.

    CAS  Google Scholar 

  8. Lanata, C F, Kaper, J. B., Baldml, M. M, Black, R E., and Levme, M. M (1985) Sensltlvlty and specificity of DNA probes with the stool blot technique for detection of Escherzchza coli enterotoxins. J Infect Dis 152, 1087–1090.

    PubMed  CAS  Google Scholar 

  9. Levine, M M., Ferreccio, C, Prado, V., Cayazzo, M, Abrego, P, Martinez, J., Maggl, L, Baldml, M. M, Martin, W, Maneval, D, Kay, B, Guers, L, Llor, H, Wasserman, S S., and Nataro, J. P (1993) Epldemiologlc studies of Escherzchzacoli diarrheal infections in a low socloeconomlc level pen-urban community in Santiago, Chile Am J Epzdemzol 138, 849–869

    CAS  Google Scholar 

  10. Rademaker, C M A., Martinez-Martmez, L., Perea, E J, Jansze, M., Fluit, A.C., Glerum, J.H., and Verhoef, J (1993) Detection of enterovirulent Escherichia coliassociated with diarrhoea in Seville, Southern Spain, with non-radtoacttve DNAprobes.J Med Microbiol 38, 87–89.

    Article  PubMed  CAS  Google Scholar 

  11. Begaud, E and Germani, Y. (1992) Detection of enterotoxigemc Escherichia coliin faecal specimens by acetylaminofluorene-labelled DNA probes Res Microbiol 143, 315–325.

    Article  PubMed  CAS  Google Scholar 

  12. Schultsz, C, Pool, G. J, VanKetel, R., De Wever, B., Speelman, P, and Dankert, J (1994) Detection of enterotoxtgemc Escherichia coli in stool samples by usingnonradioactively labeled oligonucleotide DNA probes and PCR J ClinMicrobiol 32, 2393–2391.

    CAS  Google Scholar 

  13. Stacy-Phlpps, S., Mecca, JJ, and Weiss, J B (1995) Multiplex PCR assayand simple preparation method for stool specimens detect enterotoxtgemcEscherichia coli DNA during course of infection J. Clin Microbiol 33, 1054–1059

    Google Scholar 

  14. Abdul, A A., Faruque, S. M., Ahmad, Q. S, Hossain, K M, Mahalanabis, D.,and Albert, M J (1994) Evaluation of a non-radtoactlve chemiluminescentmethod for using oligonucleotide and polynucleotide probes to identifyenterotoxtgenic Escherichia co1i J Dear Dis. Res 12, 113–116.

    Google Scholar 

  15. Jablonski, E., Moomaw, E W, Tullis, R.H, and Ruth, J. L. (1987) Prepration ofoligodeoxynucleotide-alkaline phosphatase conugates and their use ashyrbidtzation probes Nucleic Acids Res. 14, 6115–6128.

    Article  Google Scholar 

  16. Sommerfelt, H., Bhan, M. K., Snvastava, R, and Bhatnagar, S (1993) Evaluationof DNA-DNA hybrldtzatton for the direct detection of enterotoxigenic Escherichia coli in stool blots. Stand. J infect. Dis.25, 457–463

    CAS  Google Scholar 

  17. Tormeporth, N. G., John, J, Salgado, K., DeJesus, P, Latham, E., Melo, M. C.N., Gunzburg, S. T., and Riley, L. W.(1995) Differentiation of pathogenicEscherichia coli strains in Brazilian children by PCR J Clin Microbiol 33, 1371–1374.

    Google Scholar 

  18. Lang, A. L, Tsar, Y.-L, Mayer, C. L., Patton, K. C., and Palmer, C. J (1994) Multiplex PCR for detection of the heat-labile toxin gene and Shiga-like toxin Iand II genes in Escherichia coli isolated from natural waters. Appl Environ.Microbiol. 60, 3145–3149.

    PubMed  CAS  Google Scholar 

  19. Olsvik, 0. and Strockbme, N. A. (1993) PCR detection of heat-stable, heat-labile,and Shiga-like toxin genes in Escherichia coli, in Diagnostic Molecular Microbiology Principles and Applications, (Persmg, D. H., Smith, T F., Tenover, F C.,and White, T. J., eds.), Rochester, NY, Mayo Foundation, pp. 271–276.

    Google Scholar 

  20. du Ton, R., Victor, T C, and Van Helden, P. D. (1993) Empirical evaluation ofcondmons influencing the polymerase chain reaction enterotoxigemc Escheri-chia co1i as a test case. Eur J Clin Chem. 31, 225–231.

    Google Scholar 

  21. Robins-Browne, R. M. (1987) Traditional enteropathogemc Escherichia coli ofinfantile diarrhea Rev Infect Dis 9, 28–53.

    Article  PubMed  CAS  Google Scholar 

  22. Donnenberg, M. S and Kaper, J B. (1992) Enteropathogenic Escherichia coliInfect Immun 60, 3953–3961

    CAS  Google Scholar 

  23. McDaniel, T K, Jarvis, K G, Donnenberg, M S, and Kaper, J. B (1995) Agenetic locus of enterocyte effacement conserved among diverse enterobacterialpathogens Proc Nat1 Acad Sci USA 92, 1664–1668

    Article  CAS  Google Scholar 

  24. Giron, J A., Ho, A S Y., and Schoolink, G. K (1991) An inducible bundleforming pilus of enteropathogenic Escherdia coli Science 254, 710–713

    CAS  Google Scholar 

  25. Baldini, M M, Nataro, J P., and Kaper, J. B (1986) Localization of a determinant for HEp-2 adherence by enteropathogenic Escherichia cob. Infect Immun 52, 334–336.

    PubMed  CAS  Google Scholar 

  26. Nataro, J P, Baldmr, M M, Kaper, J B., Black, R E, Bravo, N., and Levine, M M. (1985) Detection of an adherence factor of enteropathogenic Escherichia coliwith a DNA probe J Infect Dis 152, 560–565

    PubMed  CAS  Google Scholar 

  27. Nataro, J. P, Maher, K O., Mackie, P, and Kaper, J. B (1987) Characterizationof plasmids encoding the adherence factor of enteropathogenic Escherichia coliInfect Immun 55, 2370–2377

    CAS  Google Scholar 

  28. Jerse, A. E, Martin, W C., Galen, J E., and Kaper, J B (1990) Oligonucleotideprobe for detection of the enteropathogenic Escherichia co1i (EPEC) adherencefactor of localized adherent EPEC.J Clin Microbiol 28, 2842–2844.

    PubMed  CAS  Google Scholar 

  29. Donnenberg, M S., Giron, J A., Nataro, J P, and Kaper, J B (1992) Aplasmidencoded type IV fimbrial gene of enteropathogenic Escherichia coli associatedwith localized adherence Mol Microbiol 6, 3427–3437

    Article  PubMed  CAS  Google Scholar 

  30. Zhang, H.-Z, Lory, S, and Donnenberg, M. S (1994) A plasmid-encoded prepilinpeptidase gene from enteropathogenic Escherichia coli.J Bacteriol 176, 6885–6891.

    PubMed  CAS  Google Scholar 

  31. Gtron, J. A, Donnenberg, M. S, Martin, W C, Jarvis, K G, and Kaper, J B (1993) Distribution of the bundle-forming pilus structural gene (bfpA) amongenteropathogenic Escherichia coli. J Infect Dis 168, 1037–1041.

    Google Scholar 

  32. Jerse, A. E, Gicquelais, K, and Kaper, J. B. (1991) Plasmid and chromosomalelements involved in the pathogenesis of attaching and effacing Escherichia coliInfect Immun 59, 3869–3815

    CAS  Google Scholar 

  33. Schmidt, H., Russmann, H, and Karch, H. (1993) Virulence determinants innontoxinogenic Escherichia coli O157 strains that cause infantile diarrhea Infect immun 61, 4894–4898

    PubMed  CAS  Google Scholar 

  34. Schmtdt, H, Russman, H, Schwarzkopf, A., Aleksic, S, Heesemen, J, and Karch, H (1994) Prevalence of attaching and effacing Escherichia coli in stool samples from patients and controls. Int J Med Microbiol Virol Parasitol Infect. Dis 281, 201–213

    Google Scholar 

  35. Franke, J., Franke, S., Schmtdt, H., Schwarzkopf, A., Wieler, L H., BalJer, G, Beutm, L., and Karch, H (1994) Nucleotide sequence analysis of enteropathogenicEscherichia coli (EPEC) adherence factor probe and development of PCR for rapiddetection of EPEC harboring virulence plasmids J Clin Microbiol 32, 2460–2463

    PubMed  CAS  Google Scholar 

  36. Gunzberg, S T, Tornieporth, N G, and Riley, L W (1995) Identification ofenteropathogenic Escherichia co1i by PCR-based detection of the bundle-formingpilus gene J Clin Microbiol 33, 1375–1377

    Google Scholar 

  37. Donnenberg, M. S., Tzipori, S., McKee, M. L., O’Brien, A. D., Alroy, J, and Kaper,J. B (1993) The role of the eae gene of enterohemorrhagic Escherichia colin in intimate attachment in vitro and in a porcine model J. Clin Invest 92, 1418–1424.

    Article  PubMed  CAS  Google Scholar 

  38. O’Brien, A D, Tesh, V. L, Donohue-Rolfe, A., Jackson, M P, Olsnes, S.,Sandvig, K., Lindberg, A A, and Keusch, G T (1992) Shiga toxin Biochemistry, genetics, mode of action, and role in pathogenesis Curr Top MicrobiolImmunol 180, 65–94

    Google Scholar 

  39. Karch, H, Heesemann, J, Laufs, R., O’Brien, A D, Tacket, C O, and Levine, M M (1987) A plasmid of Enterohemorrhagic Escherichia coli O157″H7 isrequired for expression of a new fimbrial antigen and for adhesion to epithelialcells Infect Immun. 55, 455–461

    PubMed  CAS  Google Scholar 

  40. Karch, H. and Meyer, T (1989) Evaluation of ohgonucleotide probes for tdentification of Shiga-like-toxin-producing Escherichia coli. J Clin Microbiol 27, 1180–1186.

    PubMed  CAS  Google Scholar 

  41. Levine, M M., Xu, J, Kaper, J B, Lior, H, Prado, V, Tall, B, Nataro, J, Karch, H, and Wachsmuth, K (1987) A DNA probe to identify EnterohemorrhagicEscherichia coliof 0157 H7 and other serotypes that cause hemorrhagic coltisand hemolytic uremic syndrome J Infect Dis 156, 175–182

    PubMed  CAS  Google Scholar 

  42. Gunzer, F., Bohm, H., Russman, H, Bitzan, M., Aleksic, S., and Karch, H (1992) Molecular detection of sorbitol-fermenting Escherichia coli 0157 in patients withhemolytic-uremic syndrome J Clin Microbiol 30, 1807–1810

    PubMed  CAS  Google Scholar 

  43. Schmidt, H, Karch, H, and Beutin, L (1994) The large-sized plasmids of enterohemorrhagic Escherichia coli 0157 strains encode hemolysins which are presumably members of the E cali-hemolysin family. FEMS Microbiol Lett 117, 189–196

    PubMed  CAS  Google Scholar 

  44. Brown, J. E, Sethabutr, O, Jackson, M P, Lolekha, S, and Echeverria, P (1989) Hybridization of Escherichia coli producing Shiga-like toxin I, Shtga-like toxinII, and a variant of Shiga-like toxin II with synthetic oligonucleotide probes InfectImmun 57, 281l–2814.

    Google Scholar 

  45. Karch, H, Janetzkt-Mittman, C., Aleksic, S., and Datz, M (1996) Isolation ofenterohemorrhagic Escherichia co1i 0157 strains from patients with hemolyttcuremic syndrome by usmg immunomagnetic separation, DNA-based methods, anddirect culture J Clin Microbiol 34, 516–519

    PubMed  CAS  Google Scholar 

  46. Brian, M J., Frosolono, M., Murray, B., Miranda, A, Lopez, E. L., Gomez, H F, and Cleary, TG (1992) Polymerase chain reaction for dtagnosis ofenterohemorrhagic Escherichia coliinfectton and hemolytic uremic syndrome. JClin Microbiol 30, 1801–1806

    CAS  Google Scholar 

  47. Gannon, V. P J, Rashed, M, King, R. K., and Thomas, E J G (1993) Detectionand characterization of the eae gene of shiga-like toxin-producing Escherichiacoli using polymerase chain reaction. J Clin Microbiol 31, 1268–1274

    PubMed  CAS  Google Scholar 

  48. Fratamico, P M, Sachtey, S K., Wiedmann, M., and Deng, M. Y (1995) Detectionof Escherichia coli 0157 ∶H7 by multiplex PCR. J. Clin Microbiol 33, 2188–2191

    PubMed  CAS  Google Scholar 

  49. Savarino, S. J. (1993) Enteroadherent Escherichia coli: A heterogeneous group ofE coliimplicated as diarrhoeal pathogens Trans Roy Soc Trop. Med Hyg 87 Suppl. 3, 49–53

    Article  Google Scholar 

  50. Smith, H R, Scotland, S M, Willshaw, G A., Rowe, B, Cravioto, A, and Eslava, C. (1994) Isolates of Escherichia coli O44 H18 of diverse origin areenteroaggregative J infect Dis 170, 1610–1613

    PubMed  CAS  Google Scholar 

  51. Wanke, C A, Schorling, J. B, Barrett, L J., Desouza, M. A, and Guerrant, R L (1991) Potential role of adherence traits of Escherichia coli in persistent diarrheain an urban Brazilian slum.Pediatr Infect Dis J 10, 746–751

    Article  PubMed  CAS  Google Scholar 

  52. Cravioto, A Tello, A, Navarro, A, Ruiz, J., Villafan, H., Uribe, F, and Eslava, C. (1991) Association of Escherichia coli HEp-2 adherence patterns with typeand duration of diarrhoea Lancet 337, 262–264

    Article  PubMed  CAS  Google Scholar 

  53. Eslava, C, Villaseca, J, Morales, R., Navarro, A, and Cravioto, A (1993) Identification of a protein with toxlgenic activity produced by enteroaggregative?? Escherichia coli Abstr Gen Meet Amer Sot Microbiol B105, 44. (Abstract)

    Google Scholar 

  54. Savarino, S J, Fasano, A, Watson, J, Martin, B M., Levine, M. M, Guandalini, S., and Guerry, P. (1993) Enteroaggregative Escherichia coli heat-stable enterotoxin 1 represents another subfamily of E coli heat-stable toxin Proc Natl Acad Sci USA 90, 3093–3097.

    Article  PubMed  CAS  Google Scholar 

  55. Savarino, S. J., Fasano, A., Robertson, D C., and Levme, M M (1991) Enteroaggregative Escherichia coli elaborate a heat-stable enterotoxin demonstrable in an i vitro rabbit Intestinal model J Clin Invest 87, 1450–1455

    Article  PubMed  CAS  Google Scholar 

  56. Nataro, J. P, Yikang, D, Cookson, S, Cravloto, A., Savarino, S J, Guers, L D, Levine, M M, and Tacket, C 0 (1995) Heterogeneity of enteroaggregativeEscherichia coli virulence demonstrated in volunteers. J Infect Dis 171, 465–468

    PubMed  CAS  Google Scholar 

  57. Nataro, J. P., Deng, Y, Maneval, D. R., German, A. L, Martin, W C, and Levine, M M (1992) Aggregative adherence fimbriae I of enteroaggregative Escherichia coli mediate adherence to HEp-2 cells and hemagglutination of human erythrocytes Infect Immun 60, 2297–2304

    PubMed  CAS  Google Scholar 

  58. Fang, G. D., Lima, A. M., Martin, C C, Barrett, L J, Nataro, J P, and Guerrant, R. L (1992) Aggregative HEp-2 cell adherent Escherichia coli andCryptosporidium Important pathogens in hospitalzed children with persistentdiarrhea in Northeast Brazil Abstr 32nd Intersci Conf Antimicrob AgentsChemother Abstr 686, (Abstract)

    Google Scholar 

  59. Baudry, B, Savarino, S. J, Vial, P., Kaper, J. B., and Levine, M M (1990) Asensitive and specific DNA probe to identify enteroaggregative Escherichia coli,a recently discovered diarrhea1 pathogen J Infect Dis 161, 1249–1251

    PubMed  CAS  Google Scholar 

  60. Vial, P A, Robins Browne, R, Lior, H, Prado, V, Kaper, J B, Nataro, J P, Maneval, D, Elsayed, A, and Levine, M M (1988) Characterization ofenteroadherent-aggregative Escherichia coli, a putative agent of dlarrheal disease J Infect Dis 158, 70–79.

    PubMed  CAS  Google Scholar 

  61. Kang, G, Mathan, M.M., and Mathan, V. I (1995) Evaulation of a simplifiedHEp-2 cell adherence assay for Escherichia coli isolated from south Indian children with acute diarrhea and controls. J Clin. Microbiol 33, 2204–2205

    PubMed  CAS  Google Scholar 

  62. Schmidt, H, Knop, C, Franke, S, Aleksic S, Heeseman, J, and Karch, H. (1995) Development of PCR for screening of enteroaggregative Escherichia coli J Clin.Microbiol 33, 701–705

    CAS  Google Scholar 

  63. Acheson, D W. K. and Keusch, G T. (1995) Shigella and enteroinvasive Escherichiacoli, in Infections of the Gastrointestinal Tract,(Blaser, M. J., Srmth, P. D., Ravdin, J I, Greenberg, H. B, and Guerrant, R L, eds.), New York, Raven, pp. 763–784

    Google Scholar 

  64. Goldberg, M B and Sansonetti, P J. (1993) Shigella subversion of the cellularcytoskeleton a strategy for epithelial colonization. Innfect. Immun 61, 4941–4946

    CAS  Google Scholar 

  65. Gomes, T, A. T., Toledo, R F, Trabulsi, L. R., Wood, P.K, and Morrrs, J G (1987) DNA probes for identification of enteroinvasive Escherichia coli. J. ClinMicrobiol 25, 2025–2027.

    CAS  Google Scholar 

  66. Wood, P.K., Morris, J. G, Small, P L., Sethabutr, O, Toledo, M R, Trabulsi, L., and Kaper, J B. (1986) Comparison of DNA probes and the Sereny test foridentification of invasive Shigella and Escherichia co1i strains. J Clin Microbiol 24, 498–500

    PubMed  CAS  Google Scholar 

  67. Schoolnik, G.K. (1993) PCR detection of Shigella species and enteroinvasiveEscherichia coli, in Diagnostic Molecular Microbiology. Principles and Applications, (Persmg, D H, Smith, T F, Tenover, F. C, and White, T. J., eds.), Rochester, NY, Mayo Foundation, pp.277–281.

    Google Scholar 

  68. Small, P. L. and Falkow, S. (1986) Development of a DNA probe for the virulence plasmid of Shigella spp. and enteroinvasive Escherichia coli, in Microbiology-1986, (Lieve, L, Bonventre, P. F., Morello, J A., Silver, SD., and Wu, WC., eds.), Washington, DC, American Society for Micrrobiology, pp 12l–124

    Google Scholar 

  69. Frankel, G, Riley, L, Giron, J. A., Valmassoi, J., Friedman, A., Strockbine, N., Falkow, S, and Schoolnik, G A (1990) Detection of Shigella in feces using DNAamplification. J Infect Dis 161, 1252–1256.

    PubMed  CAS  Google Scholar 

  70. Frankel, G, Giron, J. A., Vlamossoi, J, and Schoolnik, G (1989) Multi-geneamplification: simultaneous detection of three virulence genes in diarrhea1 stool Mol Microbiol 3, 1729–1734.

    Article  PubMed  CAS  Google Scholar 

  71. Gunzburg, S. T., Chang, B J, Elliott, S. J, Burke, V., and Gracey, M (1993) Diffuse and enteroaggregative patterns of adherence of enteric Escherichia coliisolated from aboriginal children from the Kimberley region of Western Australia J Infect Dis 167, 755–758.

    PubMed  CAS  Google Scholar 

  72. Bilge, S S., Apostol, J M, Jr, Aldape, M A., and Moseley, S L (1993) mRNAprocessing Independent of RNase III and RNase E in the expression of the F1845fimbrial adhesin of Escherichia coli Proc. Natl. Acad Sei USA 90, 1455–1459

    Article  CAS  Google Scholar 

  73. Yamamoto, T., Kaneko, M, Changchawalt, S., Serichantalergs, o, IJum, S., and Echeverria, P. (1994) Actin accumulation associated with clustered and localizedadherence in Escherichia coli isolated from patients with diarrhea Infect Immun 62, 2917–2929.

    PubMed  CAS  Google Scholar 

  74. Nataro, J. P., Kaper, J B, Robins Browne, R, Prado, V., Vial, P., and Levine, M M (1987) Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells Pediatr Infect Dis J 6, 829–831.

    Article  PubMed  CAS  Google Scholar 

  75. Bilge, S. S., Apostol, J. M., Jr, Fullner, K. J., and Moseley, S L (1993) Tran-scriptional organization of the F1845 fimbrial adhesin determinant of Escherichiacoli Mol Microbiol. 7, 993–1006.

    Article  CAS  Google Scholar 

  76. Nataro, J. P (1995) Enteroaggregative and diffusely adherent Escherichiacoli, in Infections of the Gastrointestinal Tract, (Blaser, M J, Smith, P D, Ravdin, J. I, Greenberg, H B, and Guerrant, R. L., eds), New York, Raven, pp 727–737

    Google Scholar 

  77. Taylor, D N and Echeverria, P (1993) Diarrhoeal disease current concepts and future chellenges. Molecular biological approaches to the epidedemiology of drarrhoealdiseases in developing countries (Review). Tras Roy Soc Trop Med Hyg 87, 3–5

    Article  Google Scholar 

  78. Levine, M M., Nataro, J P., Karch, H, Baldini, M M, Kaper, J B, Black, R E, Clements, M. L, and O’Brien, A D (1985) The diarrhea1 response of humans tosome classtc serotypes of enteropathogenic Escherichia coli is dependent on aplasmid encoding an enteroadhesiveness factor J Infect Dis 152, 550–559

    PubMed  CAS  Google Scholar 

  79. Gicquelais, K G., Baldini, M. M, Martinez, J., Maggi, L, Martin, W C, Prado, V, Kaper, J B., and Levine, M M (1990) Practical and economical method forusing biotinylated DNA probes with bacterial colony blots to identify diarrheacausing Escherichia-Coli J Cli Microbiol 28, 2485–2490.

    CAS  Google Scholar 

  80. Murray, B E., Mathewson, J J., DuPont, H L, and Hill, W E. (1987) Utility ofoligodeoxyribonucleotide probes for detecting enterotoxigenic Escherichia coliJ Infect Dis 155, 809–811

    CAS  Google Scholar 

  81. Willshaw, G A, Scotland, S M, Smith, H. R., Cheasty, T., Thomas, A, and Rowe, B. (1994) Hybidization of strains of Escherichia coli 0157 with probesderived from the eaeA gene of enteropathogenic E. colt and the eaeA homologfrom a Vero cytotoxin-producing strain of E co1i 0157 J Clin Microbiol 32, 897–902.

    PubMed  CAS  Google Scholar 

  82. Newland, J. W and Neill, R J (1988) DNA probes for Shiga-like toxins I and II and for toxin-converting bacteriophages. J Clin Microbiol 26, 1292–1297

    PubMed  CAS  Google Scholar 

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  1. Center for Vaccine Development, University of Maryland, School of Medicine, Baltimore, MD

    James P. Nataro & Juan Martinez

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Nataro, J.P., Martinez, J. (1998). Diagnosisand Investigation of Diarrheagenic Escherichia coli . In: Woodford, N., Johnson, A.P. (eds) Molecular Bacteriology. Methods in Molecular Medicine™, vol 15. Humana Press. https://doi.org/10.1385/0-89603-498-4:387

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  • DOI: https://doi.org/10.1385/0-89603-498-4:387

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