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An Approach Combining Rapid cDNA Amplification and Chemical Synthesis for the Identification of Novel, Cathelicidin-Derived, Antimicrobial Peptides

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Antibacterial Peptide Protocols

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 78))

Abstract

Host defense in mammals involves a cooperative effort by a variety of antimicrobial peptides (1), which are produced by both phagocyte precursors and some epithelia. They are called on to combat invading pathogens at sites of infection and to help protect mucosal surfaces. Although sharing some common properties, such as a high content of basic residues, these peptides vary widely in both size and primary structure.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Biophysics, and Macromolecular Chemistry, University of Trieste, Italy

    Alessandro Tossi, Marco Scocchi, Paola Storici & Domenico Romeo

  2. National Laboratory of the Interuniversity Biotechnology Consortium, AREA Science Park, Trieste, Italy

    Marco Scocchi & Margherita Zanetti

  3. Department of Biomedical Science and Technology, University of Udine, Italy

    Margherita Zanetti & Renato Gennaro

Authors
  1. Alessandro Tossi
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  2. Marco Scocchi
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  3. Margherita Zanetti
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  4. Renato Gennaro
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  5. Paola Storici
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  6. Domenico Romeo
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Editor information

Editors and Affiliations

  1. Emory University School of Medicine, Atlanta, GA

    William M. Shafer

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© 1997 Humana Press Inc.

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Tossi, A., Scocchi, M., Zanetti, M., Gennaro, R., Storici, P., Romeo, D. (1997). An Approach Combining Rapid cDNA Amplification and Chemical Synthesis for the Identification of Novel, Cathelicidin-Derived, Antimicrobial Peptides. In: Shafer, W.M. (eds) Antibacterial Peptide Protocols. Methods In Molecular Biology™, vol 78. Humana Press. https://doi.org/10.1385/0-89603-408-9:133

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  • DOI: https://doi.org/10.1385/0-89603-408-9:133

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-408-2

  • Online ISBN: 978-1-59259-564-8

  • eBook Packages: Springer Protocols

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