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Phenylboronate-chitosan resins for adsorption of β-amylase from soybean extracts

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Abstract

Isolation and purification of bioproducts from crude extracts can be obtained by affinity methods based on reversible binding of a specific molecule to ligand immobilized in a porous matrix. In the present work, nicrospheres based on chitosan matrix, which incorporated aminophenylboronic acid as a derivative, were prepared and characterized, aimed at developing a β-amylase adsorption process. Kinetic curves and adsorption isotheriru of the crude extracts as well as the breakthrough curves for a frontal chromatographic separation method of a commercial sample of β-amylase from soybean are presented. These results were compared to similar data obtained with a comercial microspheres gel based-on agarose.

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Authors and Affiliations

  1. Pharmacy and Biochemistry, Dom Bosco Catholic University, UCDB, C.P. 100, CEP 79117-900, Campo Grande, MS, Brazil

    Eduardo J. Arruda

  2. School of Chemical Engineering, DPB, UNICAMP, C.P. 6066, CEP 13083-970, Campinas, SP, Brazil

    Cesar C. Santana

Authors
  1. Eduardo J. Arruda
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  2. Cesar C. Santana
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Correspondence to Cesar C. Santana.

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Arruda, E.J., Santana, C.C. Phenylboronate-chitosan resins for adsorption of β-amylase from soybean extracts. Appl Biochem Biotechnol 108, 829–842 (2003). https://doi.org/10.1385/ABAB:108:1-3:829

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  • DOI: https://doi.org/10.1385/ABAB:108:1-3:829

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