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Characterization of a bioactive 15 kDa fragment produced by proteolytic cleavage of chicken growth hormone

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Abstract

There is evidence for a cleaved form of GH in the chicken pituitary gland. A 25 kDa band of immunoreactive-(ir-)GH, as well as the 22 kDa monomeric form and some oligomeric forms were observed when purified GH or fresh pituitary extract were subjected to SDS-PAGE under nonreducing conditions. Under reducing conditions, the 25 kDa ir-GH was no longer observed, being replaced by a 15 kDa band, consistent with reduction of the disulfide bridges of the cleaved form. The type of protease involved was investigated using exogenous proteases and monomeric cGH. Cleaved forms of chicken GH were generated by thrombin or collagenase. The site of cleavage was found in position Arg133-Gly134 as revealed by sequencing the fragments produced. The NH2-terminal sequence of 40 amino acid residues in the 15 kDa form was identical to that of the rcGH and analysis of the remaining 7 kDa fragment showed an exact identity with positions 134–140 of cGH structure. The thrombin cleaved GH and the 15 kDa form showed reduced activity (0.8% and 0.5% of GH, respectively) in a radioreceptor assay employing a chicken liver membrane preparation. However, this fragment had a clear bioactivity in an angiogenic bioassay and was capable to inhibit the activity of deiodinase type III in the chicken liver.

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  1. Carlos Arámburo

    Present address: College of Agriculture, Iowa State University, 50011, Ames, IA, U.S.A.

Authors and Affiliations

  1. Centro de Neurobiologia, Campus UNAM-UAQ Juriquilla, Universidad Nacional Autónoma de México, 76001, Querétaro, Qro., México

    Carlos Arámburo, Martha Carranza, Marisa Reyes, Maricela Luna, Hilda Martínez-Coria & Laura Berúmen

  2. Department of Animal Sciences, Rutgers-The State University of New Jersey, 08903, New Brunswick, NJ, U.S.A.

    Colin G. Scanes

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Arámburo, C., Carranza, M., Reyes, M. et al. Characterization of a bioactive 15 kDa fragment produced by proteolytic cleavage of chicken growth hormone. Endocr 15, 231–240 (2001). https://doi.org/10.1385/ENDO:15:2:231

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