Abstract
Method developments are described for a iodine speciation method. This method is based on a reversed-phase liquid chromatography separation combined with inductively coupled plasma-mass spectrometry detection. Investigations are performed concerning the methanol tolerance/stability of inductively coupled plasma, post-column oxidation/volatilization of iodine from species, influences of buffer pH, and column temperature on separation efficiency. The experiments focused on six iodine species, namely iodine, mono-iodothyrosine, di-iodothyrosine, tri-iodothyronine, reversed tri-iodothyronine, and thyroxin. Detection limits were determined between 0.08 and 1.5 µg/L (iodine related), dependent on species. Appplications on a urine sample were performed, showing iodide as the predominant iodine species.
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Michalke, B., Schramel, P. & Witte, H. Method developments for iodine speciation by reversed-phase liquid chromatography-ICP-mass spectrometry. Biol Trace Elem Res 78, 67–79 (2000). https://doi.org/10.1385/BTER:78:1-3:67
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DOI: https://doi.org/10.1385/BTER:78:1-3:67