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Sorghum (Sorghum bicolor L.)

  • Protocol
Book cover Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 343))

Summary

This chapter describes a stepwise protocol for Agrobacterium-mediated sorghum genetic transformation. Immature embryos from sorghum plants were used as the target explants. The Agrobacterium strain LBA4404, carrying a “super-binary” vector, was used in this protocol. Agrobacterium co-transformation vectors, one T-DNA containing the selectable marker gene and another T-DNA containing the trait gene(s), were also introduced in sorghum transformation for eliminating the selectable marker gene in the resulting transgenic plants. This chapter provides recommendations for analysis of the transgenic plants to confirm T-DNA integration into the sorghum genome and segregation of the selectable marker gene from the trait gene(s).

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Authors
  1. Zuo-yu Zhao
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Editors and Affiliations

  1. Center for Plant Transformation, Plant Science Institute, and Department of Agronomy, Iowa State University, Ames, Iowa, USA

    Kan Wang

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© 2006 Humana Press Inc.

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Zhao, Zy. (2006). Sorghum (Sorghum bicolor L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 343. Humana Press. https://doi.org/10.1385/1-59745-130-4:233

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  • DOI: https://doi.org/10.1385/1-59745-130-4:233

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-536-1

  • Online ISBN: 978-1-59745-130-7

  • eBook Packages: Springer Protocols

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