Abstract
Screening conditions should simulate the final application as closely as possible. This is especially a challenge when chromophore-free (e.g., aliphatic) substrates are used and no simple and reliable high-throughput method for quantitative analysis of the respective reaction product is available. The screening procedure should also be generally applicable for a certain class of enzymes. Generation of “surrogate substrates” by derivatization with chromogenic groups can have a negative effect on enzyme development by directed evolution, e.g., by causing a change in substrate specificity.
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Glieder, A., Meinhold, P. (2003). High-Throughput Screens Based on NAD(P)H Depletion. In: Arnold, F.H., Georgiou, G. (eds) Directed Enzyme Evolution. Methods in Molecular Biology™, vol 230. Humana Press. https://doi.org/10.1385/1-59259-396-8:157
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DOI: https://doi.org/10.1385/1-59259-396-8:157
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