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Isolation, Culture, and Identification of Meningococci from Clinical Specimens

  • Protocol
Meningococcal Disease

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 67))

Abstract

Humans are the only natural reservoir for meningococci. The appropriate specimens that should be taken for isolation of meningococci are dependent on the clinical question. The most appropriate specimen and/or laboratory techniques for microbiological diagnosis in an acutely sick patient with suspected invasive disease like meningitis/septicemia (1) may be quite different from those required for diagnosis of the cause of a local infection in eye, upper respiratory tract, lower respiratory tract, or urogenital tract, or for the study of the carrier state of healthy persons.

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References

  1. van Deuren M., Brandtzaeg P., and van der Meer J. W. M. (2000) Update on meningococcal disease with emphasis on pathogenesis and clinical management. Clin. Microbiol. Rev. 13, 144–166.

    Article  PubMed  Google Scholar 

  2. Danielsson D., Nathorst-Windahl G., and Saldén T. (1971) Use of immunofluo-rescence for identification of Haemophilus influenzae and Neisseria meningitidis in postmortem human tissue. Ann. NYAcad. Sci. 177, 23–31.

    Article  CAS  Google Scholar 

  3. Danielsson D. and Forsum U. (1975) Diagnosis of Neisseria infections by defined immunofluorescence. Methodologic aspects and applications. Ann. NY Acad. Sci. 254, 334–349.

    Article  CAS  PubMed  Google Scholar 

  4. Weinstein M. P. (1996) Current blood culture methods and systems: clinical concepts, technology, and interpretation of results. Clin. Inf. Dis. 23, 40–46.

    CAS  Google Scholar 

  5. Mylotte J. M. and Tayara A. (2000) Blood cultures: clinical aspects and controversies. Eur. J. Clin. Microbiol. Infect. Dis. 19, 157–163.

    Article  CAS  PubMed  Google Scholar 

  6. Centers for Disease Control and Prevention (1998) Laboratory methods for the diagnosis of meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae. Available via Internet http://www.cdc.gov/ncidod/dbmd/diseaseinfo/menigitis_manual.pdf

  7. World Health Organization (1999) Laboratory methods for the diagnosis of meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae. WHO/CDS/CSR/EDC/99.7.

    Google Scholar 

  8. Cartwright K. A. V. (ed.) (1995) Meningococcal disease. John Wiley and Sons. Chichester, UK.

    Google Scholar 

  9. Stephenson T. (1998) Coning may occur without lumbar puncture being done. BMJ 316, 1015.

    CAS  PubMed  Google Scholar 

  10. Olcén P. (1978) Serological methods for rapid diagnosis of Haemophilus influenzae, Neisseria meningitidis and Streptococcus pneumoniae in cerebrospi-nal fluid: a comparison of co-agglutination, immunofluorescence and immuno-electrophoresis. Scand. J. Infect. Dis. 10, 283–289.

    PubMed  Google Scholar 

  11. Ajello G. W., Feeley J. C., Hayes P. S., Reingold A. L., Bolan G., Broome C. V., and Phillips C. J. (1984) Trans-Isolate Medium: a new medium for primary culturing and transport of Neisseria meningitidis, Streptococcus pneumoniae and Heamophilus influenzae. J. Clin. Microbiol. 20, 55–58.

    CAS  PubMed  Google Scholar 

  12. Olcén P., Kjellander J., Danielsson D., and Linquist B. L. (1979) Culture diagnosis of meningococcal carriers. J. Clin. Path. 32, 1222–1225.

    Article  PubMed  Google Scholar 

  13. Abramson J. S. and Spika J. S. (1985) Persistence of Neisseria meningitidis in the upper respiratory tract after intravenous antibiotic therapy for systemic meningococcal disease. J. Infect. Dis. 151, 370–371.

    CAS  PubMed  Google Scholar 

  14. Cartwright K., Reilly S., White D., and Stuart J. (1992) Early treatment with parenteral penicillin in meningococcal disease. BMJ 305, 143–147.

    Article  CAS  PubMed  Google Scholar 

  15. Gästrin B., Kallings L. O., and Marcetic A. (1968) The survival time for different bacteria in various transport media. Acta Pathol. Microbiol. Scand. 74, 371–380.

    Article  PubMed  Google Scholar 

  16. Kellog J. A. and Manzella J. P. (1986) Detection of group A streptococci in the laboratory or physician’ office. Culture vs antibody methods. JAMA 255, 2638–2642.

    Article  Google Scholar 

  17. van Deuren M., van Dijke B. J, Koopman R. J., Horrevorts A. M., Meis J. F., Santman F. N. et al. (1993) Rapid diagnosis of acute meningococcal infection by needle aspiration of skin lesions. BMJ 306, 1229–1232.

    Article  PubMed  Google Scholar 

  18. Periappuram M., Taylor M. R. H., and Keane C. T. (1995) Rapid detection of meningococci from petechiae in acute meningococcal infection. J. Infect. 31, 201–203.

    Article  CAS  PubMed  Google Scholar 

  19. Kronvall G. and Myhre E. (1977) Differential staining of bacteria in clinical specimens using acridine orange buffered at low pH. Acta Pathol. Microbiol. Scand. 85, 249–254.

    CAS  Google Scholar 

  20. Hagman M., Forslin L., Moi H., and Danielsson D. (1991) Neisseria meningitidis in specimens from urogenital sites. Is increased awareness necessary? Sex. Transm. Dis. 18, 228–232.

    Article  CAS  PubMed  Google Scholar 

  21. Koppes G. M., Ellenbogen C., and Gebhart R. J. (1977) Group Y meningococcal disease in United States Air Force recruits. Am. J. Med. 62, 661–666.

    Article  CAS  PubMed  Google Scholar 

  22. Weigtman N. C. and Johnstone D. J. (1999) Three cases of pneumonia due to Neisseria meningitidis, including serogroup W-135. Eur. J. Clin. Microbiol. Infect. Dis. 18, 456–458.

    Article  Google Scholar 

  23. Odegaard A. (1983) Unusual manifestations of meningococcal infection. A review. NIPHAnn. 6, 59–63.

    CAS  Google Scholar 

  24. Isenberg H. D. (ed.) (1992) Clinical Microbiology Procedures Handbook. American Society for Microbiology Washington, DC.

    Google Scholar 

  25. Murray P. R. (ed.) (1999) Manual of Clinical Microbiology, 7th ed. ASM Press Washington, DC.

    Google Scholar 

  26. Kellog D. S. Jr. and Turner E. M. (1973) Rapid fermentation confirmation of Neisseria gonorrhoeae. Appl. Microbiol. 25, 550–552.

    Google Scholar 

  27. Olcén P., Danielsson D., and Kjellander J. (1978) Laboratory identification of pathogenic Neisseria with special regard to atypical strains: an evaluation of sugar degradation, immunofluorescence and co-agglutination tests. Acta Pathol. Microbiol. Scand. 86, 327–334.

    Google Scholar 

  28. Gomez-Herruz P., González-Palacios R., Romanyk J., Cuadros J. A., and Ena J. (1995) Evaluation of the Etest for penicillin susceptibility testing of Neisseria meningitidis. Diagn. Microbiol. Infect. Dis. 21, 115–117.

    Article  CAS  PubMed  Google Scholar 

  29. O’Callaghan C. H., Morris A., Kirby S. M., and Shingler A. H. (1972) Novel method for detection of β-lactamase by using a chromogenic cephalosporin substrate. Antimicrob. Agents Chemother. 1, 283–288.

    PubMed  Google Scholar 

  30. Bäckman A., Orvelid P., Vazquez J. A., Sköld O., and Olcén P. (2000) Complete sequence of a β-lactamase-encoding plasmid in Neisseria meningitidis. Antimicrob. Agents Chemother. 44, 210–212.

    Article  PubMed  Google Scholar 

  31. Orvelid P., Bäckman A., and Olcén P. (1999) PCR identification of a group A Neisseria meningitidis gene in cerebrospinal fluid. Scand. J. Infect. Dis. 31, 481–483.

    Article  CAS  PubMed  Google Scholar 

  32. Borrow R., Claus H., Guiver M., Smart L., Jones D. M., Kaczmarski L. B., Frosch M., and Fox A. J. (1997) Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase (siaD) PCRELISA. Epidemiol. Infect. 118, 111–117.

    Article  CAS  PubMed  Google Scholar 

  33. Borrow R., Claus H., Chaudhry U., Guiver M., Kaczmarski L. B., Frosch M., and Fox A. J. (1998) siaD PCR ELISA for confirmation and identification of serogroup Y and W135 meningococcal infections. FEMS Microbiol. Lett. 159, 209–214.

    Article  CAS  PubMed  Google Scholar 

  34. Salih M. A. M., Ahmed H. S., Hofvander Y., Danielsson D., and Olcén P. (1989) Rapid diagnosis of bacterial meningitis by an enzyme immunoassay of cerebrospinal fluid. Epidemiol. Infect. 103, 301–310.

    Article  CAS  PubMed  Google Scholar 

  35. Salih M. A. M., Ahmed A. A., Ahmed H. S., and Olcén P. (1995) An ELISA assay for the rapid diagnosis of acute bacterial meningitis. Ann. Trop. Paediatr. 15, 273–278.

    CAS  PubMed  Google Scholar 

  36. RÅdström P., Bäckman A., Qian N., Kragsbjerg P., PÅhlson C., and Olcén P. (1994) Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and streptococci using a seminested PCR strategy. J. Clin. Microbiol. 32, 2738–2744.

    PubMed  Google Scholar 

  37. Olcén P., Lantz P.-G., Bäckman A., and RÅdström P. (1995) Rapid diagnosis of bacterial meningitidis by a seminested PCR strategy. Scand. J. Infect. Dis. 27, 537–539.

    Article  PubMed  Google Scholar 

  38. Bäckman A., Lantz P.-G., RÅdström P., and Olcén P. (1999) Evaluation of an extended diagnostic PCR assay for rapid detection and verification of bacterial meningitis in CSF and other biological samples. Mol. Cell Probes 13, 49–60.

    Article  PubMed  Google Scholar 

  39. Danielsson D. and Johannisson G. (1973) Culture diagnosis of gonorrhoea. A comparison of the yield with selective and non-selective gonococcal culture media inoculated in the clinic and after transport of specimens. Acta Derm. Venereol. (Stockh) 53, 75–80.

    CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Clinical Microbiology & Immunology, Örebro Medical Centre Hospital, Örebro, Sweden

    Hans Fredlund

Authors
  1. Per Olcén
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  2. Hans Fredlund
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Editor information

Editors and Affiliations

  1. BC Research Institute for Children’s and Women’s Health, Vancouver, BC, Canada

    Andrew J. Pollard MD, PhD

  2. Wellcome Trust Centre for the Epidemiology of Infectious Disease, Oxford, UK

    Martin C. J. Maiden PhD

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© 2001 Humana Press Inc., Totowa, NJ

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Olcén, P., Fredlund, H. (2001). Isolation, Culture, and Identification of Meningococci from Clinical Specimens. In: Walker, J.M., Pollard, A.J., Maiden, M.C.J. (eds) Meningococcal Disease. Methods in Molecular Medicine™, vol 67. Humana Press. https://doi.org/10.1385/1-59259-149-3:9

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  • DOI: https://doi.org/10.1385/1-59259-149-3:9

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-849-3

  • Online ISBN: 978-1-59259-149-7

  • eBook Packages: Springer Protocols

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