Abstract
Capillary electrophoresis (CE) has become an alternative to slab-gel electrophoresis for DNA separations due to its many advantages such as speed, increased separation efficiency, requires minute amount of sample, and automation of sample loading (1) Currently, high-throughput DNA sequencing is performed exclusively by slab-gel electrophoresis coupled to fluorescence detection systems. However, slab-gel electrophoresis involves labor-intensive steps such as gel pouring, sample loading, and long electrophoretic run times. These disadvantages reduce the overall throughput efficiency of a slab-gel system making it less than ideal. One of the main goals of the Human Genome and other large scale sequencing projects is to increase the throughput rate with a commensurate reduction in the cost-per-base sequenced. A limitation of CE has been that only one capillary could be run and monitored at a time, so that the total experimental throughput is no better than with slower slab-gel system with multiple lanes. However, the recent introduction of the multiple capillary instruments, such as the ABI Prism 3700 DNA Analyzer (2) and the MegaBACE (3), may have the potential to meet the high throughput demand.
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Madabhushi, R. (2001). DNA Sequencing in Noncovalently Coated Capillaries Using Low Viscosity Polymer Solutions. In: Mitchelson, K.R., Cheng, J. (eds) Capillary Electrophoresis of Nucleic Acids. Methods in Molecular Biology™, vol 163. Humana Press. https://doi.org/10.1385/1-59259-116-7:309
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DOI: https://doi.org/10.1385/1-59259-116-7:309
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