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Cryopreservation of Avian Spermatozoa

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Cryopreservation and Freeze-Drying Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 38))

Abstract

Modern animal and plant cell cryopreservation technology began when Polge and colleagues demonstrated the cryoprotective action of glycerol by producing live chicks from domestic fowl hens inseminated with spermatozoa that had been frozen to −79°C (1). Since then there have been many reports extending these findings to other species of poultry and nondomesticated birds; describing alternative freezing/thawing protocols, cryopreservatives, diluents, and insemination regimes; and demonstrating or proposing applications of the technique to the poultry industry (see refs. 2–6 for reviews).

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References

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Author information

Authors and Affiliations

  1. Department of Molecular and Life Sciences, University of Albertay Dundee, Scotland, UK

    Graham J. Wishart

Authors
  1. Graham J. Wishart
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Editor information

Editors and Affiliations

  1. The Windermere Laboratory, Institute of Freshwater Ecology, Cumbria, UK

    John G. Day

  2. Acer Environmental, Cheshire, UK

    Michael W. Pennington

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© 1995 Humana Press Inc.

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Wishart, G.J. (1995). Cryopreservation of Avian Spermatozoa. In: Day, J.G., Pennington, M.W. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology™, vol 38. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-296-5:167

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  • DOI: https://doi.org/10.1385/0-89603-296-5:167

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-296-5

  • Online ISBN: 978-1-59259-525-9

  • eBook Packages: Springer Protocols

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