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Grassland and Turf. 2014, (02)

Effects of nitrogen forms on growth,nodulation and nitrogen-fixation of alfalfa at floweringstageChinese Full Text

YE Fang;LIU Xiao-jing;ZHANG Jin-xia;College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S.Centers for Grazingland Ecosystem Sustainability;

Abstract: Under the condition of complete nutrient solution,the effect of three kinds of nitrogen forms(nitrate nitrogen,ammonium nitrogen and nitrate nitrogen,ammonium nitrogen was 1∶1and three nitrogen levels（0,105,210mg/L）on growth nodulation and nitrogen-fixation of alfalfa were studied at flowering stage by sand culture method.The results showed that different nitrogen treatments on plant height,biomass,nodule number,nodule weight,nitrogenase activity and total nitrogen content of alfalfa were significantly higher than those of CK.Under the same nitrogen level,the plant height was the highest and higher under the effect of NO3--N and NH4+-N mixed culture and under NO3--N next,and NH4+-N culture.The performance of biomass,nodule number,nodule weight,nitrogenase activity and total nitrogen content were the best under NO3--N and NH4+-N mixed culture,it was the better and lowest under NH4+-N and NO3--N culture respectively.The performance of stem leaf ratio was the best under NO3--N training,it was better under NH4+-N training than that of NO3--N and NH4+-N mixed culture.There was significant difference in nitrogen forms between treatments（P＜0.05）.Along with the increase of nitrogen level,the plant height,biomass,nodule number,nodule weight,nitrogenase activity and total nitrogen content of alfalfa were increased,the leaf stem ratio was decreased.When the concentration of NO3--N+NH4+-N was 210mg/L,the plant height,biomass,nodule number,nitrogenase activity and total nitrogen content of alfalfa reached the maximum value,the leaf stem ratio had a minimum value.The nodule weight reached the maximum value when the concentration of NO3--N+NH4+-N was 105mg/L.In conclusion,alfalfa grows best in mixed nitrogen culture.
Keywords：

alfalfa; nitrogen forms; growth; root nodule; nitrogenase activity;

  • DOI：

    10.13817/j.cnki.cyycp.2014.02.001

  • Series：

  • Subject：

  • Classification Code：

    S541.1

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