Chest
Clinical Investigations: LavageHuman Neutrophil Collagenase (MMP-8), Identified in Bronchiectasis BAL Fluid, Correlates With Severity of Disease
Section snippets
Methods
Thirty-six patients with bronchiectasis referred to the Department of Lung Diseases of Tartu University (Estonia) were included in this study. The clinical diagnosis was based on the prolonged history of frequent bronchitis and/or pneumonia and intermittent sputum production, the persistence of crackles on auscultation, and cystic changes on CT and/or bronchograms.
Patients were divided into three different disease activity subgroups based on the clinical characteristics and lung CT findings:
Functional Activities of Collagenases in BALF of Patients With Bronchiectasis and Healthy Control Subjects
Collagenolytic activities, obtained after optimal organomercurial (PMC) activation (Fig 1) and autoactive (Fig 2) collagenolytic activities were determined after 48 h incubation of the fluid obtained by BAL (nBE=36 and nHC=l5), and 1.2 μM type I collagen with and without 1 mM PMC, respectively. Collagenolytic activities, obtained after organomercurial activation, differed significantly in different BE subgroups. The corresponding data were the following: in mild cases, 0.32×10−7 IU/L/48 h or
Discussion
Development of bronchiectases during the ongoing lung inflammation is considered to be associated with an excess of catalytically active proteinases1, 2, 3, 4, 5 in target tissues. Circulating inflammatory cells, polymorphonuclear leukocytes (PMNs), and monocytes,21, 45 as well as pulmonary resident cells, fibroblasts, epithelial cells, and alveolar macrophages, synthesize and release various proteinases.17, 19, 20 Activation of such locally released proteinases exerts the potential to result
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Supported by Academy of Finland and Ministry of Social and Health Affairs of Finland.
revision accepted October 18.