FOXO regulates the expression of antimicrobial peptides and promotes phagocytosis of hemocytes in shrimp antibacterial immunity
Fig 4
FOXO translocated into nucleus in shrimp challenged by V. anguillarum.
(A, B) The FOXO distribution patterns in the cytoplasm (A) and nucleus (B) of intestine cells of shrimp challenged by bacteria, as analyzed using western blotting (lower panels of A and B). The western blotting bands were digitalized using ImageJ software by scanning the bands from three repeats. The relative expression levels of FOXO/β-actin or FOXO/Histone 3 were expressed as the mean ± SD, and the value of the control shrimp was set as one. (C) The nuclear translocation of FOXO in hemocytes of shrimp 2 h post V. anguillarum challenge was detected using fluorescent immunocytochemical assays. PBS injection was used as control. Scale bar = 20 μm. (C’) Statistic analysis of panel C. the colocalization of FOXO and DAPI-stained nuclei in hemocytes was analyzed by WCIF ImageJ software. (D) The bacterial number in the hemolymph and intestines of Foxo-knockdown shrimp followed V. anguillarum injection; dsGfp-injection in shrimp following bacterial injection was used as control. (E) The survival rate of Foxo-RNAi shrimp infected by V. anguillarum. dsGfp injection was used as the control.