GAS6 signaling tempers Th17 development in patients with multiple sclerosis and helminth infection
Fig 1
Helminth infection differentially modulates TAM receptor and their ligands expression in patients with MS and type 2 environment.
A) Representative dot plots showing the three main sub-populations of mononuclear cells based on the expression of CD11b, CD4, and CD1c. TYRO3, AXL, and MERTK expression were evaluated in circulating monocytes (CD11bhighCD4mid) and DCs (CD1chighCD11blow) of patients with MS, patients with HIMS, and healthy controls by flow cytometry. B-G) The percentage of TYRO3-positive as well as the mean fluorescent intensity (MFI) of the receptor on monocytes (B, C, and D) and DCs (E, F, and G) are graphed. H, I, and J) The percentage of AXLhigh as well as the MFI of the receptor on DCs are shown. K, L, and M) The expression of MERTK on DCs is shown as both percentage and MFI. N, O, and P) The percentage of circulating CD4+ T cell expressing GAS6 as well as its MFI are shown. Q, R, and S) The percentage of circulating CD4+ T cell expressing PROS1 and its MFI are shown. Data is presented as a pool of independent samples included in the specific staining (Control N = 21–31; MS N = 10–27; and HIMS N = 11–16). One-way ANOVA with a Fisher post hoc test was performed to determine statistical significances, *p<0.05 **p≤0.01 ****p≤0.001. MS = multiple sclerosis, HIMS = helminth-infected multiple sclerosis.