EBNA2-deleted Epstein-Barr virus (EBV) isolate, P3HR1, causes Hodgkin-like lymphomas and diffuse large B cell lymphomas with type II and Wp-restricted latency types in humanized mice
Fig 8
P3HR1 infected lymphomas express both Wp-restricted and type II viral latency proteins and the immediate-early lytic protein BZLF1.
A. Immunoblot analysis of proteins isolated from P3HR1 infected, AG876 infected or B95.8 virus infected lymphomas was performed using antibodies against EBNA1, EBNA2, EBNA3A (EBV latency protein expressed in type III and Wp-restricted latency), LMP1 (EBV latency protein expressed in type II and III latency), LMP2A (EBV latency protein expressed in type II and type III latency), lytic protein BZLF1 or alpha tubulin (loading control). P3HR1 “1” protein is isolated from mouse #1 and P3HR1 “2” protein isolated from mouse #2 in S1 Table. EBNA-LP protein as expected can be expressed in multiple different sizes, and EBNA2 protein as expected is larger in type 1 EBV B95.8 infected tumors compared to type 2 EBV AG876 infected tumors. B. IHC co-staining studies were performed using antibodies that detect LMP1 or BZLF1 in a P3HR1 infected tumor (mouse #2 in S1 Table). No co-staining was observed. Blue arrow shows an example of an EBNA-LP staining cell, and brown arrow shows an example of an LMP1 positive cell. C. IHC co-staining studies were performed on a P3HR1 infected tumor using antibodies that detect BZLF1 or CD138. (mouse #2 in S1 Table). Blue arrow shows an example of a BZLF1 only staining cell, brown arrow shows an example of a CD138 only staining cell, and red arrows show examples of BZLF1/CD138 co-staining cells.