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Murine polyomavirus DNA transitions through spatially distinct nuclear replication subdomains during infection

Fig 6

pRPA32S4S8 and pATMS1981 localization is disrupted in NG18 VRCs.

MEFs were infected with NG18 (ΔMT/ΔST), then processed and labeled as described in Fig 2, then imaged by 3D-SIM. (A) Cells were stained for RPA32 (green), LT (red), and pRPA32S4S8 (gray). Arrowhead indicates region of pRPA32S4S8 localization relative to RPA32 and LT. (B) PCC analysis of the pRPA32S4S8 signal with combined, dim, or focal RPA32 subsets in WT- and NG18-infected cells. (C) PCC analysis of LT with pRPA32S4S8. n = 10 nuclei. (D) Cells were stained for pATMS1981 (gray) and either total RPA32 (green) or LT (red). Protein combinations were labeled and imaged separately due to incompatible antibody sources. (E) PCC analysis of pATMS1981 with combined, dim, or focal RPA32 subsets in WT- and NG18-infected cells. (F) PCC analysis of LT with pATMS1981. n = 12 nuclei. Single z-planes of representative nuclei are shown. Scale Bars: Nucleus = 5μm, Crop = 0.5μm. Unpaired t-tests were used to compare mean values (*** = p<0.001; ** = p<0.01; ns = p>0.05).

Fig 6

doi: https://doi.org/10.1371/journal.ppat.1008403.g006