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The oxidative fumarase FumC is a key contributor for E. coli fitness under iron-limitation and during UTI

Fig 10

Absence of FumC leads to decreased susceptibility to bactericidal antibiotics.

Wild-type CFT073 and the oxidative and reductive TCA pathway mutant strains were examined in LB medium containing (A) chloramphenicol 10 μg/ml (B) trimethoprim 5 μg/ml or (C) tetracycline 10 μg/ml. Samples were collected over the course of 180 minutes, diluted and plated on LB agar to determine CFU. Colored dots signify the mean of three biological replicates. The Minimum Bacterial Concentration (MBC) of (D) ciprofloxacin 0.002–32 μg/ml (E) ampicillin 0.016–256 μg/ml or (F) streptomycin 0.064–24 μg/ml observed for wild-type CFT073 and the mutant strains were recorded following overnight incubation on Mueller-Hinton agar with antibiotic test strips. The values shown are averages from two independent trials.

Fig 10

doi: https://doi.org/10.1371/journal.ppat.1008382.g010