Amblyomma americanum ticks utilizes countervailing pro and anti-inflammatory proteins to evade host defense

Feeding and transmission of tick-borne disease (TBD) agents by ticks are facilitated by tick saliva proteins (TSP). Thus, defining functional roles of TSPs in tick evasion is expected to reveal potential targets in tick-antigen based vaccines to prevent TBD infections. This study describes two types of Amblyomma americanum TSPs: those that are similar to LPS activate macrophage (MΦ) to express pro-inflammation (PI) markers and another set that suppresses PI marker expression by activated MΦ. We show that similar to LPS, three recombinant (r) A. americanum insulin-like growth factor binding-related proteins (rAamIGFBP-rP1, rAamIGFBP-rP6S, and rAamIGFBP-rP6L), hereafter designated as PI-rTSPs, stimulated both PBMC -derived MΦ and mice RAW 267.4 MΦ to express PI co-stimulatory markers, CD40, CD80, and CD86 and cytokines, TNFα, IL-1, and IL-6. In contrast, two A. americanum tick saliva serine protease inhibitors (serpins), AAS27 and AAS41, hereafter designated as anti-inflammatory (AI) rTSPs, on their own did not affect MΦ function or suppress expression of PI markers, but enhanced expression of AI cytokines (IL-10 and TGFβ) in MΦ that were pre-activated by LPS or PI-rTSPs. Mice paw edema test demonstrated that in vitro validated PI- and AI-rTSPs are functional in vivo since injection of HEK293-expressed PI-rTSPs (individually or as a cocktail) induced edema comparable to carrageenan-induced edema and was characterized by upregulation of CD40, CD80, CD86, TNF-α, IL-1, IL-6, and chemokines: CXCL1, CCL2, CCL3, CCL5, and CCL11, whereas the AI-rTSPs (individually and cocktail) were suppressive. We propose that the tick may utilize countervailing PI and AI TSPs to regulate evasion of host immune defenses whereby TSPs such as rAamIGFBP-rPs activate host immune cells and proteins such as AAS27 and AAS41 suppress the activated immune cells.


Introduction
Ticks are among the most important ecto-parasites with global public and veterinary health impact. In terms of diversity of transmitted disease pathogens, ticks far outpace any known vector arthropod, and are considered second to mosquitoes in terms of impact of the transmitted disease pathogens. In the livestock industry, losses due to ticks and tick-borne diseases (TBD) are estimated to be worth millions of US dollars annually [1]. Globally, the impact of TBD in public health has been on the rise, with the food-for-thought article on "One Health" listing several TBDs among sources of human health concerns needing One Health solutions [2]. Similarly, of the 23 human vector-borne diseases (VBD) that were listed by the World Health Organization, seven are TBD agents: Crimean-Congo hemorrhagic fever, Lyme disease, relapsing fever (borreliosis), rickettsial diseases (spotted fever and Q fever), tick-borne encephalitis, and tularemia (http://www.who.int/mediacentre/factsheets/fs387/en/). In the United States, the Centers for Disease Control (CDC) listed 16 human TBD agents (http://www.cdc. gov/ticks/diseases), six of which were transmitted by Ixodes scapularis and four by Amblyomma americanum ticks. Additionally, six human TBD agents were listed on the 2018 National Notifiable human VBD in the USA and its territories. In fact, from 2004 to 2016, the six human TBDs accounted for nearly 77% of human VBDs in the USA and its territories [3].
In the absence of effective vaccines against major TBD agents, controlling ticks using acaricides remains the only method to protect animals and humans against TBD infections [4]. Serious limitations such as environmental contamination and ticks developing resistance that threaten acaricide-based tick control have justified the need to develop alternative tick control methods [5][6][7][8]. Immunization of animals against tick feeding has been validated as an alternative method [9]. The approach is attractive because it is environmentally friendly and is postulated to be effective against both susceptible and acaricide-resistant tick populations. The limiting step is the availability of effective vaccine antigens. Except for a few instances when human TBD infections occurred after exposure to contaminated materials [10][11][12][13][14][15][16], transmission of both animal and human TBD agents occur during tick feeding. Thus, a deeper understanding of how the tick accomplishes feeding is a rational approach through which vaccine targets can be identified [17][18][19][20][21].
Host inflammatory response is the first line of defense against the tick feeding style of disrupting host tissue and sucking up blood. Macrophages (MF), which have been confirmed to infiltrate the tick feeding site [22][23][24][25] are among key effector cells of the inflammatory response. Importantly, MF act as a bridge between innate and adaptive immunity [26,27]. From this perspective, it is logical that parasites including ticks might target MF to evade host immune defenses. A limited number of studies have documented immuno-modulatory effects against MF functions by mostly yet undefined tick salivary factors. Salivary gland protein extracts (SGE) of fully fed Rhipicephalus microplus suppressed expression of pro-inflammatory (PI) co-stimulatory markers CD86 and CD69, but not CD40 and CD80 by LPS-activated MF (92). The same authors found that R. microplus SGE suppressed secretion of PI cytokines by LPS activated MF [28]. Likewise, SGE of D. variabilis inhibited secretion of PI cytokines by LPS-activated MF [29]. In this same study, D. variabilis tick saliva proteins stimulated MF to secrete copious amounts of prostaglandin 2, which in turn stimulated fibroblast migration. In a related study, D. variabilis tick saliva was shown to increase both basal and platelet-derived growth factor stimulated migration of MF [30]. In another study, Amblyomma variegatum tick saliva proteins were shown to suppress expression of MHC-II, CD40, CD80, IL-12-p40, and TNF-α, but increased IL-10 expression by MF [31]. Similar immuno-modulatory effects against MF functions were also reported in Rhipicephalus sanguineus [32]. These studies clearly indicated that ticks target MF functions in their quest to evade host immune defenses and facilitate feeding and transmission of TBD agents.
At the time of drafting this manuscript few defined tick proteins that modulated MF functions were reported including a pro-inflammatory MF migration inhibitory factor has been described in ticks including A. americanum [33][34][35][36], but function was not validated. In another study, Ixodes scapularis cystatin was shown to affect MF function in response to Anaplasma phagocytophilum infection [37]. Here, we report that similar to LPS, A. americanum insulin-like growth factor binding protein-related proteins (AamIGFBP-rP1, AamIGFBP-rP6L, and AamIGFBP-rP6S) [38, 39] stimulated MF to express PI markers. Interestingly, we also show that A. americanum serine protease (AAS) inhibitors (serpin)-27 and 41 [40], blocked LPS and AamIGFBP-rPs activated MF to express PI markers. We have described our findings with reference to understanding molecular mechanisms that regulate tick feeding.
We further analyzed the effects on MF expression of PI cytokines (Fig 3). Furthermore, ELISA results determined the expression of PI cytokines showed that rAamIGFBP-rP1-and rAamIGFBP-rP6L-treated MF induced expression of TNF-α and IL-6 ( Fig 3). The effects of rAamIGFBP-rP1 and AamIGFBP-rP6L displayed a dichotomous effect on TNFα expression: whereas transcription was not affected (Fig 3A and 3C), it was secreted at significant levels in high dose rAamIGFBP-rP1 ( Fig 3B) and low dose rAamIGFBP-rP6L ( Fig 3D). In rAa-mIGFBP-rP6S treated MF, the effect was opposite: the high dose induced significant TNF-α transcript but had no effect on its secretion (Fig 3F). In the case of IL-6, both rAamIGFBP-rP1 and AamIGFBP-rP6L apparently induced transcription and secretion (Fig 3G, 3H, 3I and 3J), while rAamIGFBP-rP6S had not effect (Fig 3K and 3L).
We next tested the synergistic effect of the three PI-rTSPs as a cocktail (Fig 4). It is interesting to note that secretion levels of TNF-α, IL-1, and IL-6, were statistically similar to levels that were induced by LPS activated of MF (Fig 4A, 4B and 4C). Treatment of MF with the PI-rTSP cocktail might have over activated the cells in that AI cytokines, IL-10 ( Fig 4D) and TGFβ ( Fig  4E) were also significantly induced.  Next, we tested the effects of PI-rTSPs cocktail on MF morphology ( Fig 4F). Confocal microscopy revealed that PI-rTSPs activated MF had the morphology that was similar to LPS activated MF as indicated by stretched spindle-like shaped cells compared to smooth and round cells in the media only control ( Fig 4F). Altogether, results from the in vitro study confirmed that the PI-rTSPs stimulated MF to express PI markers.
We next investigated the effects of the AI-rTSP cocktail on expression of cytokines by MF that were first activated by LPS or PI-rTSPs (Fig 7). Except for TNFα, which was apparently suppressed, but not significantly (Fig 7A), there was no effect on IL-1, IL-6, and IL-10 expression by LPS-activated MF (Fig 7B, 7C and 7D). In Fig 7E, the AI-rTSP cocktail moderately but not significantly enhanced secretion of TGFβ in LPS activated MF. With exception of TNF-α, which was significantly suppressed by the highest dose (Fig 7F), expression of IL-1, IL-6, IL-10, and TGFβ were enhanced when PI-rTSP activated MF were treated with the AI-rTSP cocktail (Fig 7G, 7H, 7I and 7J).

PI-rTSP and AI-rTSP differentially regulated inflammatory markers in mice paws
Effects of PI-rTSPs and AI-rTSPs on inflammation marker expression were investigated by qPCR (Figs 9-11). Consistent with cell culture data (Fig 2), PI co-stimulatory markers, CD40, CD80, and CD86 were up regulated in mice that were injected with low dose individual PI- Median fluorescent intensity levels of co-stimulatory markers CD40, CD80 and CD86, on RAW MF treated with LPS or (5B, 1-9) with various doses of PI-rTSPs followed by AAS27 and AAS41 cocktail. Filled gray chromatogram = isotype control, dark gray bar graph = control (media treated cells); Blue line chromatogram and bar = MF that were pre-activated by LPS or PI-rTSP followed by treatment with AAS27 and AAS41 cocktail; Red line chromatogram and bar = MF treated with LPS or PI-rTSP only. Please note that, histograms are representative of one treatment. Graph inserts are mean of three biological replicates and presented as absorbance values ± SEM. Arrows (#) indicate percentage (%) decrease in CD40, CD80 or CD86 expression.
Fig 10 summarizes expression of PI and AI cytokines in PI-rTSP induced edema. TNF-α, IL-1, and IL-6 were significantly expressed in low dose individual (Fig 10A, 10B and 10C) and PI-rTSP cocktail (Fig 10E, 10F and 10G) treatment. In contrast, except for IL-1, which was slightly enhanced (Fig 10J), expression of TNF-α and IL-6 was below negative control in animals that were injected with high dose (25 μg) of the individual PI-rTSP (Fig 10I and 10K). Except for TNF-α which was significantly up regulated above normal saline (Fig 10M), other transcripts were apparently up regulated but not significantly (Fig 10N, 10O and 10P). Co-injecting with the low dose of AI-rTSP had mixed effects on expression of the four cytokines (Fig 10E-10H). In low dose-treated mice, co-injecting AI-rTSP suppressed TNFα bỹ 35% and IL-6 by 62% (Fig 10E and 10G), but not IL-1 ( Fig 10F). Likewise, co-injecting with high dose AI-rTSP reduced expression of TNFα by 74% (Fig 10M), while IL-1 and IL-6 were apparently enhanced but not significantly (Fig 10N and 10O). Also notable, the TGFβ transcript was induced in animals that were injected with low dose of the individual PI-rTSPs ( Fig  10D) as well as the high dose of the PI-rTSP cocktail (Fig 10P), but not the low dose cocktail ( Fig 10H) and high dose individual PI-rTSP ( Fig 10L).
The effects of PI-rTSPs and AI-rTSPs on chemokine transcription mirrored cytokine expression. Notably, chemokine transcription was significantly upregulated in mice that were injected with low dose, individual (11A, B, C, D, E) or cocktail (Fig 11F, 11G, 11H and 11I) PI-rTSP with exception of CCL5, for which there was no amplification. Except for CCL5 ( Fig  11M), which was upregulated in high dose of the individual PI-rTSPs, other chemokines tested were expressed below normal saline injected controls (Fig 11J, 11K, 11L and 11N). In animals that were injected with high dose of the PI-rTSP cocktail, all chemokines were up regulated (Fig 11O, 11P, 11Q, 11R and 11S). Also, notable, co-injecting with the high dose AI-rTSP cocktail suppressed expression of CXCL1, CCL2, CCL3, and CCL11 by~50%, 53%, 65%, and 72% respectively (Fig 11O, 11P, 11Q and 11S) and CCL5 which was enhanced (Fig 11R). Amblyomma americanum ticks utilizes pro-and anti-inflammatory proteins to evade host defense

Discussion
Hard ticks successfully feed and transmit tick borne disease (TBD) agents by secreting numerous tick saliva proteins (TSPs) to thwart the host's immune defenses, which would otherwise reject the tick. Thus, discovery of tick immuno-modulatory TSPs is highly sought after as these might serve as targets in tick-antigen based vaccines to prevent TBD infections. This study provides evidence to suggest that A. americanum ticks might utilize countervailing functions of PI proteins to regulate the evasion of host immune defenses. Our in vitro and in vivo data in this study has demonstrate that AamIGFBP-rP-1, AamIGFBP-rP6S, and AamIGFBP-rP6L (38, 39) activated MF to express PI markers, whereas A. americanum AAS27 and AAS41 suppressed expression of PI markers by activated MF. This study builds on our previous studies that showed that AamIGFBP-rP-1, AamIGFBP-rP6S, and AamIGFBP-rP6L were up regulated when ticks were stimulated to start feeding [38, 41], were immunogenic and injected into the host within 24-48 h after attachment [42], and if disrupted by RNAi silencing prevented successful tick feeding [43]. Likewise, the observed AI effects of rAAS27 and rAAS41 in this study is in agreement with our recent studies that showed that AAS27 might inhibit inflammation by targeting trypsin and plasmin and AAS41 by targeting chymase and chymotrypsin [44].
Although not yet determined at the tick feeding site, activated MF at an inflamed site occur in a spectrum that is bordered by classically activated PI (M1) and alternately activated (M2) antiinflammation phenotypes [45]. Broadly, the M1 phenotype is induced at the front end of an immune reaction, and the M2 at the tail end of an immune response to resolve the inflammatory response. M1 MF express multiple factors that drive inflammation including co-stimulatory markers, CD40, CD80, and CD86, cytokines such as TNF-α, IL-1β, IL-6, and IL-12, oxygen intermediates and reactive nitrogen species such as nitric oxide (NO) [46][47][48][49][50]. On the other hand, M2 MF express factors that lead to resolution of inflammation to protect against self-injury (50). On this basis, we speculate that PI-rTSPs in this study might activate skin resident MF to the M1 phenotype to drive local inflammation, while AI-rTSPs, which enhanced expression of anti-inflammation markers might play roles in resolution or moderation of TSP-induced inflammation. The observation that both rAAS27 and rAAS41 reduced the expression of PI markers by both LPSand PI-rTSPs-activated MF was not surprising in that several studies have previously reported tick immuno-suppressive effects against skin immune cells in tick saliva [51][52][53][54][55]. However, the findings that rAAS27 and rAAS41 did not apparently affect non-activated MF, but selectively affected functions of MF that were spontaneously activated or pre-activated by LPS and PI-rTSP was intriguing. From the perspective of tick feeding physiology and tick transmission of TBD agents, data in this study raise interesting future research questions. For instance, it would be interesting to further explore the effect of rAAS27 and rAAS41 reversing MF that were pre-activated by PI-rTSP on the outcome of an immune response to TBD agents. A notable observation in our flow cytometry data was that, AI-rTSPs were much more effective against LPS-activated MF than PI-rTSP. Interestingly, TBD agents such as B. afzelii, B. burgdorferi [56,57], E. chaffeensis [58], A. phagocytophilum [59] and, Powassan virus [60] can activate MF to express high levels of PI cytokines. However, this does not seem to limit colonization of the host by transmitted TBD agents. Also, interestingly, clinical outcomes of TBD infections are more pronounced when TBD agents are co-inoculated with tick saliva proteins [31]. Given the finding that rAAS27 and rAAS41 reversed activated MF, it is possible that AI-TSPs described here could prevent MF from killing transmitted TBD agents, which ultimately could aid pathogen transmission. Several studies have reported that tick saliva/SGE of different tick species including A. variegatum, I. scapularis, R. microplus, R. appendiculatus, R. sanguineous, and D. variabilis suppressed expression of PI markers by MF that were first activated by LPS or were exposed to pathogens [29,[61][62][63][64]. Given our observations that AAS27 and AAS41 suppressed expression of pro-inflammation markers by LPS-activated MF, it is potentially possible that the immunosuppressive effects observed in tick saliva/SGE of different tick species could be mediated by AAS27 and AAS41 homologs.
Skin inflammation in response to tick bites in humans and rodents were characterized by high expression of multiple PI cytokines and chemokines [65][66][67]. On this basis, we suspect that high expression of PI cytokines and chemokines in PI-rTSP-induced edema suggests that these proteins could play roles in mediating tick and host interactions. An inflammatory response is characterized by infiltration of multiple innate immune cells including mast cells, MF, and neutrophils [41, [68][69][70]. Although we did not conduct immuno-histochemical staining and cellular imaging of inflamed paws to enumerate immune cells, the observed high expression of cytokines and chemokines, which are expressed by various immune cells possibly suggest an influx of immune cells into PI-rTSP injected paws. Specifically, the observation of high expression of PI co-stimulatory markers, CD40, CD80, and CD86, which are mostly produced by the M1 MF phenotype [71], could suggest the presence of these cells in injected paws. Chemokines that were investigated in this study were selected for their roles in attracting immune cells to the site of inflammation [72,73]. Therefore, the observed high expression of chemokines suggested that the edema formation was a result of various cellular migration at the inflamed site.
The findings in this study are not unique as pro-inflammation tick proteins including a tick histamine release factor and an 84 kDa tick serine protease, were previously reported [74,75]. Given the expectation that in order to complete feeding, ticks must evade the host's innate immune defense response against tick feeding that also include inflammation, our findings in this study may be considered counterintuitive. However, from the perspective of tick feeding physiology, inflammation can be beneficial to tick feeding as it might lead to increased blood flow into the feeding site benefiting the tick in acquiring the blood meal. Additionally, the increased flow of inflammatory cell monocytes into the inflamed tick feeding site might result in enhanced transmission of A. americanum transmitted TBD agents such as Bourbon virus, Heartland virus, and E. chaffeensis that have tropisms for inflammatory cells including MF [76,77].
It is also important to note that treating MF with high dose (10 μg) of PI-rTSP cocktail also induced expression of an anti-inflammation cytokines, IL-10, which conforms to the M2 MF phenotype. We are of the view that, the higher concentration of PI-rTSP over-stimulated MF to the extent the correct anti-inflammation response was triggered. Similarly, we also observed that, with exception of TNFα, other cytokines, IL-1, IL-6, IL-10, and TGFβ were enhanced in MF that were first treated with PI-rTSP for 24 h followed by AI-rTSP for another 24 h. Whether or not, these findings occur physiologically remains to be investigated. Like most functional analysis studies of recombinant tick saliva proteins, the limitation is that the amount of native TSPs that are injected into the host during tick feeding is unknown, and thus future studies to define functional roles of native proteins are required.
In conclusion, we have proposed that A. americanum tick might utilize countervailing functions of pro-and anti-inflammatory proteins to regulate evasion of host defenses. Our proposal is that the tick first secrets PI-TSPs that stimulate MF into the pro-host defense phenotype and then secretes AI-TSPs to de-activate the activated immune cells including MF. This proposal assumes that proteins in this study were sequentially secreted during tick feeding. The findings in this study warrants further investigations into functional roles of proteins in this study in transmission of TBD agents, and their utility as anti-tick vaccine antigens.

Ethical statement
Healthy, pathogen-free BALB/c mice were purchased from Charles River laboratories (Wilmington, MA). Animal experiments were done according to the animal use protocol (#2015-0079) approved by Texas A&M University Institutional Animal Care and Use Committee (IACUC) that meets all federal requirements in Animal Welfare Act (AWA), the Public Health Service Policy (PHS), and the Humane Care and use of laboratory animals.

Expression of A. americanum tick saliva insulin-like growth factor binding proteins-related proteins (AamIGFBP-rP) in Human Embryonic Kidney (HEK)
Preliminary assays revealed that insect-cell expressed recombinant (r) AamIGFBP-rP1,-AamIGFBP-rP6L, and AamIGFBP-rP6S (with endotoxins removed) activated both immortalized and PBMC derived macrophages (MF) to express PI markers. To rule out the possibility of endotoxin involvement in the observed results, rAamIGFBP-rP1, rAamIGFBP-rP6L, rAa-mIGFBP-rP6S rTSPs were re-expressed in Human Embryonic Kidney (HEK 293) mammalian cells using the pcDNA 3.3 expression plasmid (Thermo-Scientific). Modified pcDNA 3.3 with added CD5 secretion signal to allow secretion of the recombinant protein into spent media was kindly provided by Dr. Mwangi (Kansas State University, Kansas, USA). Mature protein encoding open reading frames was sub-cloned into the modified pcDNA3.3 plasmid using primers listed in Table 1. The reverse primer included the flag tag sequence (DYKDDDDK) which was used for detection and affinity purification of the recombinant protein. Recombinant plasmids were transformed into E.coli DH5α cells and subsequently purified using plasmid miniprep kit (Omega) followed by quantification and transfection. HEK-293A adherent cells were used in pilot expression, and the HEK-293 Freestyle (HEK-293F) cell line (Thermo-Scientific) was used for large-scale rTSP production in suspension cultures [78].
Adherent 293A cells were grown to a monolayer of up to~60-70% confluence in T-75 flasks containing 10 mL of Dulbecco's modified Eagle's medium (DMEM) (Lonza) supplemented with glutamine and heat-inactivated fetal bovine serum (10%) at 37˚C with 5% carbon dioxide (CO 2 ) and 85-90% humidity. For transfection, 1 μg of plasmid DNA and 2.4 μL of the transfection reagent, polyethyleneamine (PEI) diluted in 180 μL of Opti-MEM (Thermo-Scientific) medium was incubated for 20 min at room temperature (RT). Following the incubation, 180 μL of the plasmid DNA-PEI mixture was added to each plate containing the cells, mixed gently by rocking the plate, and incubated for~48 h at 37˚C in the humidified incubator with 5% CO 2 [79].
Expression was validated by immuno-staining of HEK cells using the monoclonal antibody to FLAG (5 μg/mL) (Sigma-Aldrich). Fast red was used to (4-Chloro-2-methylbenzenediazonium salt, Naphthol, Sigma-Aldrich) stain the cells, which were visualized using inverted microscope. Spent media was subjected to routine ELISA using the HRP (Horse-radish peroxidase) conjugated antibody to FLAG-Tag (Sigma-Aldrich) to verify secretion of the recombinant product into culture media.
For large-scale expression, HEK-293F suspension cultures were grown to mid-logarithmic phase with shaking at 125 rpm in 8% CO 2 and 85% relative humidity. The cells were then Table 1. Expression primers of Amblyomma americanum tick saliva insulin-like growth factor binding proteins-related proteins in HEK cells.

Amblyomma americanum rTSP PRIMER SEQUENCE (Restriction sites underlined, FLAG Tag and linker sequence highlighted in grey)
rAamIGFBP-rP1 seeded at 30 × 10 6 viable cells in 293 Freestyle media (Thermo-Scientific), allowed to incubate for 2 h. Recombinant plasmids (30 μg each) were incubated in 1 mL Opti-MEM media (Thermo-Scientific) separately. Following incubation, 293fectin transfection reagent (Thermo-Scientific) and recombinant plasmids were combined and incubated for an additional 30 min and then added to 30 mL cell culture containing 30 million HEK-293F cells. The cell suspension was harvested after 72 h and recombinant proteins were purified using FLAG M2 affinity gel purification (Sigma). Bound recombinant proteins were eluted in 1 mL fractions under acidic conditions using 0.1M Glycine buffer (pH 3.5) and immediately neutralized with 25 μL 1M Tris-HCl (pH 8.0). Purification was confirmed using routine SDS-PAGE and Coomassie blue or silver staining. Subsequently, relevant fractions were combined, concentrated and dialyzed against Tris-NaCl buffer (50 mM Tris, 150mM NaCl, pH 7.4) using the 10 kDa molecular cut off membrane spin filters (Pall Life Sciences). Expression recombinant AAS27 and AAS41 is reported elsewhere [44].

Macrophage cell activation assays
This was done using bovine PBMC differentiated and immortalized RAW 267 MF (ATCC).
In the preliminary assay, PBMCs were isolated from bovine peripheral blood using Ficoll-Paque solution (Sigma-Aldrich). Monocytes from the PBMCs were isolated by Magnetic sorting method using primary mouse anti-bovine CD14 (Washington State University) antibody and a secondary antibody, anti-mouse IgG microbeads (Miltenyi Biotec). The cells were labelled with the anti-CD14 antibody and then loaded onto a MACS column, for CD14 labelled monocytes. The monocytes were counted and allowed to differentiate into macrophages for 6-8 days. Cell morphology was visually confirmed using an inverted microscope (Nikon). Following differentiation, the assay was set up using individual concentrations of 10 μg /mL, 1 μg/mL and 0.1 μg/mL of insect cell expressed rAamIGFBP-rP1, rAamIGFBP-rP6L, rAamIGFBP-rP6S [39] and yeast expressed A. americanum serpin (AAS) 27 and 41 (44). Following 24 h, the cells were collected and stained for CD40, CD80, and CD86 expression and then analyzed by flow cytometry (described below). Lipopolysaccharide (LPS), a bacterial cell wall component was used as positive controls and media alone served as a negative control. All assays were performed in triplicates for each time. Subsequent to the preliminary assay, RAW 264.7 MF (ATCC) were used in the assay. Routinely, cells were grown in Dulbecco's Modified Eagle's Medium (DMEM) (Lonza) supplemented with 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, sodium bicarbonate, nonessential amino acid solution (Thermo-Scientific) and 10% fetal bovine serum (FBS) (Thermo-Scientific). Routinely, MF were seeded in 12 or 24 well plates overnight and cultured at 37˚C with 5% CO 2 , 85-90% humidity overnight to approximately 80% confluency. For pro-inflammation activation assays, 10 6 MF were incubated with 0.10, 1.0, and 10 μg/mL of affinity purified HEK cell expressed rAamIGFBP-rP1, rAamIGFBP-rP6L, and rAamIGFBP-rP6S.
In preliminary analysis, we had observed that pro-inflammation markers were expressed at below background in MF that were treated with various dosages of rAAS27 and rAAS41. To investigate this, we conducted two assays. In the first assay, expression of pro-inflammation makers was determined in MF that were co-cultured with various dosages (0.1, 1.0, 10 μg/mL of the mixture of rAAS27 or rAAS41 and LPS or rAmIGFPB-rPs. In the second assay, MF were first activated with LPS (100 ng) or various dosages (0.1, 1.0, 10 μg/mL) of rAamIGFBP-rP1, rAamIGFBP-rP6L and rAamIGFBP-rP6S for 24 h before replacing spent culture media with fresh media supplemented with various dosages (0.1, 1.0, 10 μg/mL) of individual or cocktail mix of rAAS27 and rAAS41. At 24 and 48 h post incubation, spent cultures were processed for assays described below.

Cell surface marker staining and flow cytometry
Treated and non-treated MF, were detached, and re-suspended in staining medium (DMEM with sodium azide). Immuno-labeling of bovine (preliminary analysis) and murine cell surface markers was performed by incubating cells with 15 μg/mL fluorescein isothiocyanate (FITC) conjugated antibodies to CD40 (Abcam), CD86 and CD80 (Thermo-Scientific) and isotype matched control mAbs IgG2a and IgG2b (Abcam) for 30 min on ice. After incubation, cells were washed three times with DMEM media containing 0.01% sodium azide and re-suspended in 400 μL 1X PBS (pH-7.4) containing 1 μg/mL propidium iodide, for excluding dead cells, and analyzed by flow cytometry with parameters set to 10,000 events, filter setting 530/30 nm wavelength (FACS Caliber) using acquisition software BD CellQuest (BD Biosciences) and the analysis program FlowJo 9.8.5 (TreeStar) at digital imaging core facility (College of Veterinary Medicine, Texas A&M University, College Station, TX).

Nitric oxide and Cytokine detection in the spent media
Nitric oxide metabolites released in the cell culture supernatant as nitrate or nitrite was detected by Total Nitric Oxide detection kit (Thermo-Scientific) according to the manufacturer's protocol. Photometric measurement of the absorbance due to this azo chromophore determined the NO 2 -(nitrite) concentration at 540 nm wavelength using an ELISA plate reader (BioTek Instruments). Cytokine ELISA of TNFα, IL-1, IL-6, IL-10, IL-12, and TGFβ was done using specific antibodies (Ready-SET-Go!, eBioscience, Thermo-Scientific). Optical densities were measured using an ELISA plate reader at 450 nm wavelength (BioTek Instruments, Inc).

MF phenotype staining
RAW 264.7 MF were seeded on Nunc Lab-Tek Chamber Slide (Thermo-Scientific) in the presence of the cocktail (10 μg/mL) of rAamIGFBP-rP1, rAamIGFBP-rP6L, and rAamIGFBP-rP6S, or positive control (100 ng LPS), or negative control (media with buffer) for 24 h. The cells were then fixed with ice cold methanol, blocked with 1% BSA, 22.52 mg/ml glycine in PBST (PBS+0.1% Tween 20) for 30 min and immuno-stained with the antibody to Actin (Abcam). The following day, the cells were washed in 1 X PBS and incubated with secondary antibody conjugated with Alexa Fluor 488 (Abcam) for 1 h in the dark. The cells were washed, treated with mounting media containing DAPI (Thermo-Scientific) for nuclei staining and cell staining was analyzed by confocal microscopy. Images were acquired with Ziess LSM 780 confocal microscope and merged using Zen 2012 SP1 (black edition) software in the Imaging facility (College of Veterinary Medicine, Texas A&M University, College Station, TX).

Paw edema assay
Retired female breeder BALB/c mice (Envigo) were maintained for one week to acclimatize prior to experiment. The carrageenan-induced hind paw inflammation model was used to investigate the PI and AI role of rTSPs [80]. Prior to each injection, the basal footpad volume was recorded using a plythesmometer (Harvard Apparatus). Four experiments were conducted using three mice per group. In the first experiment, effects of a high dose cocktail of rAamIGFBP-rP1, rAamIGFBP-rP6L, and rAamIGFBP-rP6S was assessed. To make the high dose cocktail, approximately 40 μg each of endotoxin free mammalian cells expressed rAa-mIGFBP-rP1, rAamIGFBP-rP6L and rAamIGFBP-rP6S were combined, and concentrated to reduce volume using Jumbosep centrifugal spin filter devices (Pall Life Sciences). Similarly, 40 μg each of endotoxin free rAAS27 and rAAS41 were combined and concentrated as above.
In the third and fourth experiments, mice were injected with low doses of cocktail of individual proteins. To make the low dose cocktail, 10 μg each of rAamIGFBP-rP1, rAamIGFBP-rP6L and rAamIGFBP-rP6S with or without 10 μg of rAAS27 and rAAS41 cocktail were prepared. In the third experiment, the first group of mice received the 10 μg the PI-rTSP cocktail, the second received the 10 μg of the AI-rTSP cocktail, the third group received 10 μg of AI-rTSP and PI-rTSP cocktail, the fourth group received 10 μg of rAAS27 or rAAS46 individually. In the fourth experiment, each group of mice received 10 μg of AamIGFBP-rP1, AamIGFBP-rP6L and AamIGFBP-rP6S. For all experiments, algae derived inflammation agonist, carrageenan (2% w/v in 0.9% saline) was used as positive control and normal saline (9 g/L NaCl) or 150mM 50mM Tris NaCl buffer (pH 7.4) as negative control ( Table 2).
As index of edema formation, the first and second experiment inflammation was measured at times 0 (before injection), 20, 40, 60, 120, 240, 360, 720, and 1440 min' post injection. For the third and fourth experiments, paw edema was measured at 0, (before injection), 20, 40, 60, and 120 or 130 min' post injection. After measurements, the mice were euthanized (3L/minute, CO 2 ) and injected and non-injected paws were placed in cryotubes and snap frozen in Amblyomma americanum ticks utilizes pro-and anti-inflammatory proteins to evade host defense liquid nitrogen. The paws were collected at the level of calcaneus bone for cytokine, chemokine, and myeloperoxidase assays.

Quantitative RT-PCR
Total RNA was routinely extracted using the TRIzol reagent according to instructions (Thermo-Scientific). From MF, cells were lysed directly into 1 mL TRIzol. For paws, tissues were minced in 1 mL TRIzol solution using sterile soft tissue scissors followed by sonication using the tissue dismembranator (VWR). Total RNA from paws were subjected to mRNA isolation using OligodT magnetic beads (Thermo-Scientific) and bound mRNA was eluted using elution buffer (10mM Tris-HCl, pH 7.5). The NanoDrop (BioTek Instruments, Inc) was used to determine quantity and quality of total RNA for cDNA synthesis. Template cDNA was synthesized from 500 ng total RNA and 25 ng mRNA using the Verso cDNA synthesis kit (Thermo-Scientific). The Verso cDNA synthesis kit contains an enhancer reagent, which prevents genomic (g) DNA carryover into the synthesized cDNA. The reverse transcription cDNA synthesis step included incubation at 42˚C from 30 min and 95˚C for 2 min in the thermocycler (Bio-Rad). After cDNA synthesis, the working stock for each sample was diluted 1:10 with DEPC treated water.
The qPCR (Quantitative polymerase chain reaction) was performed in triplicates in a 50 μl final reaction mix containing 3 μl each specific primers (300 nM each, Table 3), 5 μl 1:10 diluted template cDNA, and 25 μl 2X SYBR (Thermo-Scientific) green PCR master mix [(Applied Biosystems 7300 Real Time PCR System (Thermo-Scientific) and Bio-Rad qPCR machine (Bio-Rad)]. Settings were: 50˚C for 2 min for one cycle followed by 95˚C for 10 min, 95˚C for 40 cycles at 15 seconds' interval and 60 º C for 1 minute. Cognate mRNA expression levels were determined using the comparative delta Δ C t method [81]. The GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) gene was used as an internal reference gene. Amplifications from non-treated controls were used as calibrator for in vitro experiments and normal saline injected controls for in vivo experiments. For each biological replicate, we did qPCR in three technical triplicates.

Statistical analysis
Data acquisition for flow cytometry was performed by using BD Cell Quest (BD Bioscience). The data analysis program used was FlowJo 9.8.5 (TreeStar). The cells with >80-90% viability was selected by gating on the flow cytometer. The results from each experiment were normalized to negative control and One-way ANOVA followed by Dunnett's Post hoc test was used to determine the statistical differences between the controls and treatments. The data are represented as means ± standard error (SE) and p values of �0.05 were considered to represent statistically significant differences using Prism 8.0 (GraphPad Software Inc).