The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation
Fig 5
ISG20 co-localizes with P bodies.
A) HEK293T ISG20-expressing cells were analyzed by confocal microscopy along with two markers of P bodies, TNRC6A and DDX6 in the presence or absence of a one-hour incubation with 100 μg/ml of Puromycin that induces a translational stress known to increase the number of cells expressing P bodies as well as the number of P bodies on a per cell basis [45]. Control cells not expressing ISG20 are shown only as a zoomed overlay, while the complete panel are presented in S12B Fig. The graph presents the proportion of cells exhibiting detectable P bodies in the presence or absence of puromycin and/or ISG20. B) The different ISG20 mutants were similarly analyzed by confocal microscopy with the exception that an HA-tagged, destabilized form of TNRC6A was also concomitantly expressed by transfection to increase the extent of P bodies accumulation. Representative pictures and distributions (out of >100 cells per condition/mutant in two to three independent experiments) are shown. The graph presents the proportion of P bodies with or without ISG20 in double-positive cells.