Caspase-mediated cleavage of murine norovirus NS1/2 potentiates apoptosis and is required for persistent infection of intestinal epithelial cells
Fig 7
NS1/2 cleavage is necessary for infection of intestinal epithelial cells.
Ileum tissue from MNV-infected WT (A-C) or DKO (D-F) mice was analyzed via RNAscope at 2–3 days post-infection. MNV RNA is in red, EpCAM is in green, and nuclei are stained with DAPI in blue. The tables in A and D indicate the total number of mice analyzed and the number of mice with MNV genome-positive cells in epithelial (EpCAM-positive) or non-epithelial (EpCAM-negative) compartments. Yellow arrows in images indicate MNV+ EpCAM+ cells and white arrows indicate MNV+ EpCAM-negative cells. Data points in B and E represent individual mice and indicate the number of MNV+ EpCAM+ cells per cm of tissue. White scale bar, 20μm. Statistical significance determined by one-way ANOVA with Tukey’s multiple comparisons test. **, p ≤ 0.01; ****, p≤ 0.0001.