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Initiation, extension, and termination of RNA synthesis by a paramyxovirus polymerase

Fig 3

Primer-dependent RNA synthesis and inhibition by NiV L-P.

(a) A 12-mer template with four bases complementary to a short 4-nucleotide primer was used to analyze primer-extension activity of NiV L(wt)-P. (b) Product sizes are indicated using a 4-5-6-mer combined RNA size marker (lane M). NiV L(wt)-P, template, and primer were incubated in the presence of α33P-GTP tracer (lane 1), α33P-GTP + ATP (lane 2), α33P-GTP + ATP + CTP (lane 3), α33P-GTP + ATP + R1479-TP (lane 4), and α33P-GTP + CTP + GS-5734-TP (lane 5). (c) Total RdRp activity in primer extension and de novo formats reported after normalizing to the NiV L(wt)-P (see S7 Fig). Samples size was n = 2 and error bars represent standard deviation.

Fig 3

doi: https://doi.org/10.1371/journal.ppat.1006889.g003