A leucine aminopeptidase is involved in kinetoplast DNA segregation in Trypanosoma brucei
Fig 5
Characterization of procyclic T. brucei expressing ectopically TbLAP1-HA.
(A) Growth of trypanosomes in SDM-79 in the presence (dotted line) and absence (continuous line) of tetracycline (Tet). (B) DAPI counts in induced cells with Tet for ectopic expression of TbLAP1-HA. Two hundred cells per time point were counted and used for the analysis. (C) Western blot analysis of ectopically expressed TbLAP1-HA using monoclonal anti-tubulin antibody as a loading control. 5 x 106 cells per sample were loaded per well. (D) Mitochondrial membrane potential measurement using TMRE of uninduced (black line) and induced (red line) cells expressing TbLAP1-HA (2, 6, 24, 72 and 96 hrs of induction). The measurement is a representative of an assay performed in three independent experiments.