Functional diversity of secreted cestode Kunitz proteins: Inhibition of serine peptidases and blockade of cation channels
Fig 9
Detection of EgKU-3 and EgKU-8 in parasite secretions.
Analysis by MALDI-TOF MS of hydatid fluid from a bovine cyst (A), as well as of chymotrypsin-affinity purified fractions from the same sample (B) and from the supernatant of cultured immature adults (C). Signals whose m/z values could derive from the EgKUs are indicated (MH+ predicted for mature EgKU-3 and EgKU-8 are: 6406.8 and 6520.9, respectively). Note that the signals putatively corresponding to the EgKUs are significantly enriched in the eluate from the affinity matrix. The identity of EgKU-3 and EgKU-8 purified from cyst fluid was subsequently confirmed by peptide mass fingerprinting (see S1 Dataset and the text for further details), as previously described [3].