Transgenic Expression of the Anti-parasitic Factor TEP1 in the Malaria Mosquito Anopheles gambiae
Fig 4
P. berghei infections of mosquitoes expressing TEP1r under the Vg promoter.
a. P. berghei infection of TEP1Δct2 mutant mosquitoes rescued with transgenic TEP1r. Shown are the number of live parasites (green circles) and melanized parasites (black circles) in the midguts of mosquitoes 7 days after infection. Control mosquitoes are TEP1 mutant alone (TEP1Δct2) and transgenic mosquitoes expressing GFP instead of TEP1r (Vg-GFP). b. P. berghei infection of TEP1ΔT mutant mosquitoes compared to TEP1ΔT rescued with transgenic TEP1r (TEP1ΔT;Vg-TEP1r). c. P. berghei infection of transgenic mosquitoes expressing TEP1r in a wild type TEP1s background. Live (green circles) and melanized (black circles) parasites are compared in wild type (TEP1s) and transgenic (TEP1s;Vg-TEP1r) mosquitoes. d. P. berghei infection of transgenic TEP1r mosquitoes in wild type TEP1s background after TEP1 allele-specific knockdown. Compared are transgenic mosquitoes treated with dsLacZ, dsTEP1s and dsTEP1r, 3 days prior to infection. For all P. berghei infections, experiments were performed at least 3 times, representative experiments are shown. The raw numbers of parasites and calculated p values for all infections are provided in S2 Table.