Inhibition of Malaria Infection in Transgenic Anopheline Mosquitoes Lacking Salivary Gland Cells
Fig 1
The gene structure of the piggyBac transformation vector, pBac[pAAPP-mBax; 3xP3-EGFP], TG mosquito lines, and insertion sites.
(A) The gene construct derived from the piggyBac-based vector contains piggyBac Left-arm (L) and Right-arm (R) with an inverted terminal repeat (ITR). The T7-mBax gene is expressed under the control of the An. stephensi aapp promoter (pAAPP) and An. gambiae trypsin terminator (Tryter). The transformation marker, EGFP is expressed under the control of the 3xP3 promoter. A double line represents the probe region for a Southern blot analysis. The restriction enzyme (Msp I) site is represented below the scheme. The red arrow represents the primer sites for a RT-PCR analysis in Fig 2. (B) A Southern blot analysis of AAPP-mBax lines. Genomic DNA from AAPP-mBax mosquito lines (lines 1 and 3) was digested with Msp I, and hybridized with a fragment corresponding to the piggyBac R region. (C) Insertion sites of the transgene in AAPP-mBax lines 1 and 3. The blue bars show the local DNA region within each genomic scaffold. Black boxes represent the annotated protein-coding region in the VectorBase (https://www.vectorbase.org/). Double-headed arrows show the piggyBac construct. L: piggyBac Left-arm, R: piggyBac Right-arm.