Latency Entry of Herpes Simplex Virus 1 Is Determined by the Interaction of Its Genome with the Nuclear Environment
Fig 6
Detection of HSV-1 genomes in neurons from human TGs.
(A) Table showing the characteristics of the five patients from which TGs were analyzed. (B) PCR for the detection of HSV-1 genomes in the five TGs harvested from patient 1 to 5. TK and LAT loci were detected. The lane HSV-1 indicates viral genomes detection in infected cells used as a positive control. C is the negative control issued from uninfected cells. (C) RT-PCR for detection of several lytic transcripts and LAT in the five human TGs. C: RNA from controlled infected cells (for ICP0, 4, 27, UL30 and VP16 detection) or mouse TG (for LAT detection). RT is for Reverse Transcription. “-”and “+” indicate samples without or with reverse transcription, respectively. (D) IF for the detection of three neurofilament markers (NF160, NF200 and ßIII-tub). (E) IF for the detection of PML and neurofilaments. (F) RNA FISH for LAT detection using tyramide 488 (i, green) or tyramide 350 (ii, blue). (G) RNA-DNA FISH combined with IF for the detection of LAT transcripts (blue), HSV-1 genomes (red), and PML (green). Three different PML patterns are shown. The PML signal in non-neuronal cells is shown in (i). Scale bars represent 10 μm.