Skip to main content
Advertisement

< Back to Article

Antigenic Fingerprinting following Primary RSV Infection in Young Children Identifies Novel Antigenic Sites and Reveals Unlinked Evolution of Human Antibody Repertoires to Fusion and Attachment Glycoproteins

Fig 1

Evaluation of RSV F and G gene fragment phage display library using neutralizing monoclonal antibodies.

(A) A gene fragment library displaying random fragments and spanning the entire F gene was used to map the binding epitope of anti-F monoclonal antibodies, Palivizumab and D25. The schematic on top represents the F protein including the location of previously discovered antigenic regions: ϕ, I, II and IV. The alignments of the displayed fragments on the phages selected by Palivizumab and D25 in the F-GFPDL are shown in blue and red bars, respectively. The thickness of the bars represents the relative frequencies of the bound fragments. Numbers at the bottom represent the amino acid residues of the RSV-F protein. (B) Representation of the Palivizumab and D25 antibody epitopes identified by either X-Ray crystallography or GFPDL within the pre-fusion F protein structure (PDB Id- 4JHW). Red refers to the D25 epitope and blue represents the Palivizumab epitope. (C) A gene fragment library displaying random fragments and spanning the entire G gene was used to map the binding epitope of MAb 131-2G. Schematic represents the G protein including previously identified central conserved domain (CCD). (D) Consensus amino acid sequences identified as the epitope recognized by the monoclonal antibodies using GFPDL or other methods.

Fig 1

doi: https://doi.org/10.1371/journal.ppat.1005554.g001