Dual Requirement of Cytokine and Activation Receptor Triggering for Cytotoxic Control of Murine Cytomegalovirus by NK Cells
Fig 6
m157-specific cytotoxicity in vivo is greatly enhanced by poly(I:C) and dependent on IFN-I and degranulation.
(A-D) Mice were i.p. injected with 100 μg poly(I:C), PBS, 200 μg anti-NK1.1, 200 μg anti-Ly49H and/or isotype control. After 16 hours 2x106 differentially CFSE-labeled splenocytes were injected intravenously. Three hours after adoptive transfer splenocytes were analyzed by flow cytometry. (A) Representative histograms showing splenic CD19+CFSE+ cells. Numbers indicate percentage of m157-Tg CFSElow and WT CFSEhigh cells. (B-D) Specific lysis of m157-Tg targets. (E) Mice were injected IP with 100 ug poly(I:C) or PBS. After 16 hours the spleens were harvested and NK cells were analyzed for GzmB. Data are representative of two to three independent experiments with individual points representing a single mouse.