CD39 Expression Identifies Terminally Exhausted CD8+ T Cells
Fig 4
Transcriptional analysis of CD39+ and CD39– CD8+ T cells in HCV infection.
(A) Consensus hierarchical clustering of expression profiles from CD39+ (black) and CD39– (grey) CD8+ T cells from 8 HCV infected patients. Clustering is based on the top 10% of genes by variance across the dataset. Sample similarity (1-Pearson correlation coefficient) is annotated with color from low (white) to high (green). (B) Gene set enrichment map displaying Gene Ontology gene sets enriched (FDR < 0.1) in CD39+ CD8+ T cells from (A). Nodes (in red) are sized in proportion to gene set size; connecting line thickness represents extent of gene member overlap between gene sets. (C) Gene set enrichment analysis of a signature of 200 genes up-regulated in exhausted CD8+ T cells from the mouse model of chronic viral infection versus acute infection (day 30 post infection) in the ranked list of genes differentially expressed by CD39+ vs. CD39– CD8+ T cells. Leading edge genes are indicated by orange symbols. (D) Volcano plot of all genes (grey) or exhausted leading edge genes (orange) from (C).