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Crystal Structure of Calcium Binding Protein-5 from Entamoeba histolytica and Its Involvement in Initiation of Phagocytosis of Human Erythrocytes

Figure 7

Down regulation of EhCaBP5 reduces the rate of phagocytosis.

(A) Schematic representation of sense and anti-sense construct. EhCaBP5 was cloned in sense and anti-sense orientation in BamH1 and Kpn1 site of pEhHyg-TetR-O-CAT vector. (B, C) Western blot analysis of anti-sense (B) and Sense EhCaBP5 (C). Thirty microgram of lysate from sense and forty microgram of anti-sense cells in presence and absence of tetracycline were loaded to each lane. HM1 was used as a positive control and coactosin as a loading control. (D) RBC uptake assay performed with cells expressing sense and anti-sense constructs in the presence and absence of tetracycline. The experiment was repeated three times independently in triplicates. Comparisons were made with respect to cells vector alone or cells without addition of tetracycline. Statistical significance was determined by paired- t test. P-values for ** is P<0.001, #P>0.05.

Figure 7

doi: https://doi.org/10.1371/journal.ppat.1004532.g007