Dengue Viruses Are Enhanced by Distinct Populations of Serotype Cross-Reactive Antibodies in Human Immune Sera
Figure 2
Removal of cross-reactive antibodies from primary DENV2-immune sera, eliminates enhancement of heterotypic DENV infection in vitro.
A) DENV-binding ELISA shows that depletion of a primary DENV2-immune serum with DENV3 virus removes binding to heterotypic DENV1, DENV3 and DENV4. (D–E) In ADE assays performed in K562 cells, depletion of cross-reactive antibodies from primary DENV2-immune serum eliminated enhancement of heterotypic DENV1 (B), DENV3 (D), and DENV4 (E) but not homotypic DENV2 virus (C). Data are representative of at least four different primary DENV2-immune human sera. *Significantly different by Students t-test with p<0.01, **DENV3-depleted ADE curves significantly different from control-depleted ADE curves at serum dilutions 1∶40, 1∶80, 1∶160 and 1∶320 (Students t-test with p<0.001).