The Glycosylated Rv1860 Protein of Mycobacterium tuberculosis Inhibits Dendritic Cell Mediated TH1 and TH17 Polarization of T Cells and Abrogates Protective Immunity Conferred by BCG
Figure 8
Analysis of spleen DC ex vivo from infected mice.
Splenocytes obtained from mice uninfected (UI) or infected 12 hours earlier with 107 cfu of BCG-GFP or BCG-TB1860 were processed for flow cytometry as described in Methods and illustrated in Figure S5 in Text S1. The number of CD11c-high DC displaying upregulated expression MHC class II (A), secreting IL-2/TNF-α (B) and secreting IL-12p40 (C) are shown as a percentage of total splenocytes within gate P1 of Figure S5A. Splenocytes simultaneously secreting IL-2/TNF-α along with IL-12p40 (D) or simultaneously secreting IL-2/TNF-α along with expression of MHCII (E) are shown as a percentage of total CD11c-high DC.