Expansion of Murine Gammaherpesvirus Latently Infected B Cells Requires T Follicular Help
Figure 1
Reduced frequency of latently infected B cells in SAP-deficient mice during the establishment of latency.
Mice were infected intranasally with either 1,000(low dose) or 4×105 pfu (high dose) of MHV68-H2bYFP and splenocytes were harvested at days 16–18 post-infection. (A) Representative flow cytometry plots showing identification of virus infected (YFP positive) B cells. Flow plots were gated on CD3−, B220+ cells. (B and C) Frequency of virus infected B cells determined by YFP expression at days 16–18 post-infection following either low dose or high dose infection. Each symbol represents an individual infected mouse, and the horizontal line represents the mean frequency of virus infected B cells. ****, p<0.0001. (D and F) Limiting dilution PCR analysis to determine the frequency of viral genome positive splenocytes. (E and G) Limiting dilution analysis to determine the frequency of infected splenocytes capable of reactivating virus. Serial dilutions of splenocytes were plated on MEF monolayers, and reactivating virus was detected by the presence of cytopathic effect (CPE) 14 days after plating. Results are from 4 (B,D,E) or 2 (C,F,G) independent experiments with 3 to 5 mice per group.