Highly Active Antiretroviral Therapies Are Effective against HIV-1 Cell-to-Cell Transmission
Figure 4
Combinations of NRTIs are highly effective against HIV-1 cell-to-cell transmission.
(A, B) An experiment as in Fig. 2 was performed for the combinations (black lines) of (A) AZT and TFV and (B) 3TC and ABC and compared to each single inhibitor treatment (red lines, left and right panel). The X-axis represents the drug concentration of the drug within the combination that is being compared to the single drug treatment (in red). See Supplementary Fig. S8A for additional inhibitor combinations. (C) The change in IC90 for co-culture over cell-free infection for the single inhibitors was compared to all the inhibitor combinations tested. (D) The change in average IIP at ICMax for co-culture over cell-free infection for the single inhibitors was compared to all the inhibitor combinations tested. See Supplementary Fig. S8B for complete set of average IIP data. (E) Cell-free and co-culture infection of primary cells with HIV-1NL4-3 carrying the M184V mutation of reverse transcriptase (black line) compared to wild-type HIV-1NL4-3 (red line) in the presence of increasing concentrations of AZT. Error bars represent the standard deviation from the combination of at least two individual experiments each done in triplicate.