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Intranasal Vaccination Promotes Detrimental Th17-Mediated Immunity against Influenza Infection

Figure 5

IL-17A neutralization reduces augmented neutrophil response.

Mice vaccinated intranasally with split influenza virus antigen (A/Uruguay/716/2007 (H3N2)) and CRX-601 were administered with anti-Ly-6G or anti-IL-17A (100 µg) i.p. one day prior to challenge with influenza virus and then daily for a further 6 days post challenge. Neutrophil depletion efficiency was monitored in peripheral blood 24 h post antibody treatment (A,B). Confirmation of neutrophil depletion (C) as well as enumeration of Gr-1+ subsets (D) was examined in the lung 5 days post infection. Data in B and D are means ± SEM for three biological replicates and is representative of two independent experiments. * p<0.05, ** p<0.01, ***p<0.001, are significantly different from control, Rat IgG2a (One-way ANOVA, Dunnet post-test).

Figure 5

doi: https://doi.org/10.1371/journal.ppat.1003875.g005