CXCR3-Dependent CD4+ T Cells Are Required to Activate Inflammatory Monocytes for Defense against Intestinal Infection
Figure 3
Intestinal parasite burden is elevated in Cxcr3−/− mice.
Paraffin sections of Day 10-infected WT and Cxcr3−/− small intestines were stained by immunohistochemistry for T. gondii antigen. Shown are representative images of WT LP (A), Cxcr3−/− LP (B), WT Peyer's patch (C), and Cxcr3−/− Peyer's patch (D) with positive parasite staining in brown. (E) Lung (WT: n = 3; KO: n = 4), liver (WT: n = 5; KO: n = 4), spleen (WT: n = 5; KO: n = 4), MLN (WT: n = 3; KO: n = 5), Peyer's patches (PP, WT: n = 2, KO: n = 2) and small intestines (WT: n = 5; KO: n = 4) were harvested during acute infection, and DNA was isolated from tissues and subjected to quantitative PCR amplification for the parasite B1 gene and the host arginosuccinate lyase gene. Parasite burden was quantitated as parasite to host genome equivalents and was calculated by comparison to a standard curve obtained from known amounts of Toxoplasma. Pooled ratios are represented as mean +/− SEM where **p<0.01.