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CD36 Recruits α5β1 Integrin to Promote Cytoadherence of P. falciparum-Infected Erythrocytes

Figure 3

Inhibition of IRBC cytoadherence and adhesive force on TNF-α-stimulated HDMEC by RGD peptide.

(A) Flow cytometric analysis of the expression of CD36, ICAM-1, α5, β1 and VCAM-1 on unstimulated and TNF-α-stimulated HDMEC. TNF-α was added at 1 ng/ml for 20–24 h. Results shown are representative of at least 3 experiments. (B) Adhesion of IRBC from the parasite line 7G8 to unstimulated and TNF-α-stimulated endothelial monolayers pre-incubated with 20 µM RGD peptide or 5 µg/ml of anti-CD36 for 30 min at 37°C in 5% CO2 (n = 3). (C) Adhesion of IRBC from two clinical parasite isolates to unstimulated and TNF-α-stimulated endothelial monolayers pre-incubated with 20 µM RGD peptide, 5 µg/ml of anti-ICAM-1 or both (n = 5 with 2 clinical isolates). (D) Force of detachment measurements of 7G8 parasites on unstimulated and TNF-α-stimulated endothelial monolayers pre-incubated with 20 µM RAD or RGD peptide (n = 3). (E) Force of detachment measurements of two clinical parasites on unstimulated and TNF-α-stimulated endothelial monolayers pre-incubated with 20 µM RAD or RGD peptide (n = 3 with 2 clinical isolates).

Figure 3

doi: https://doi.org/10.1371/journal.ppat.1003590.g003