Crystal Structure of the Full-Length Japanese Encephalitis Virus NS5 Reveals a Conserved Methyltransferase-Polymerase Interface
Figure 1
Global views of JEV NS5 structure.
Structure of NS5 shown in orientations A) looking into the RdRP front channel (putative dsRNA channel); B) along the NTP entry channel; C) viewing from the top of RdRP. MTase is in cyan, RdRP palm in gray, thumb in blue, fingers in light red, N-terminal extension in pink, priming loop in purple, and signature sequence SGDD in magenta. Zinc ions are shown as brown spheres. The numbers defining the residue ranges of MTase and RdRP are shown in panel C. The three missing residues (271–273) in the linker are indicated by dashed lines in panel C. For clarity purposes, protein structures in all figures are shown in thin ribbon style with only the key β-sheet structures shown in regular cartoon style in most of the figure panels. “N-ext.” is used as abbreviation for “N-terminal extension”.