Cryotomography of Budding Influenza A Virus Reveals Filaments with Diverse Morphologies that Mostly Do Not Bear a Genome at Their Distal End
Figure 2
Time course immunofluorescence imaging of filament formation in MDCK cells infected with Influenza A/Udorn/72.
DAPI was used to stain cell nuclei (blue) while phalloidin was used to detect actin (red). Monoclonal antibodies were used to detect viral proteins; NP is shown in green and HA is shown in white. Budding virus is seen from as early as 6 hours post infection. From 8 hours we can see long filaments and Archetti bodies at the cell surface.