A Unique Spumavirus Gag N-terminal Domain with Functional Properties of Orthoretroviral Matrix and Capsid
Figure 5
Sedimentation velocity analysis of Gag-Env interface mutants.
C(S) functions that best fit sedimentation velocity profiles from (A) wt PFV-Gag-NtD, (B) V14S, (C) L17S, (D) V14S/L21S and (E) N29Q mutants. The C(S) function from 75 µM Gag-NtD (black) and from 75 µM equimolar mixtures of Gag-NtDs with Env(1–20) (red) are shown in each panel. (F) Histogram of equilibrium association constants derived from the sedimentation data as described in methods.