Caveolin-1 Protects B6129 Mice against Helicobacter pylori Gastritis
Figure 1
Cav1-deficient mice show enhanced gastritis upon infection with H. pylori SS1.
B6129 WT and Cav1-KO mice (n = 15 per group) were infected with the mouse-adapted CagA-delivery incompetent H. pylori SS1 strain for 11 month. (A) Stomachs of Cav1-KO mice were less colonized by SS1 bacteria than those of WT mice. Ex vivo quantification of colony forming units (CFUs) per mg of resected stomach tissue. The data are presented as mean ± S.E. (n = 15 per group); *p = 0.0141 KO versus WT. (B–C) Hematoxylin & Eosin (H&E) and Warthin-Starry silver (WS) stainings from paraffin sections of mouse gastric tissue were evaluated for signs of histopathology and results were scored for individual mice (0+ no, 1+ mild , 2+ moderate, 3+ severe gastritis). Data are presented as in (A) (n = 15 per group); *p = 0.0002 KO versus WT. Cav1-KO mice show active chronic gastritis with intramucosal lymphocyte follicles, foveolar hyperplasia and loss of parietal cells. Quantitative analyses (B) are shown together with representative histology (C); magnifications 100×, 200×.