Dissection of Ire1 Functions Reveals Stress Response Mechanisms Uniquely Evolved in Candida glabrata
Figure 11
Effects of IRE1 deletion on stress response in wild-type and Δcnb1 backgrounds in C. glabrata.
(A) Ire1 plays a role in azole tolerance in C. glabrata when calcineurin is absent. Logarithmic-phase cells of each C. glabrata strain were adjusted to 2×107 cells/ml, and then 5 µl of serial 10-fold dilutions were spotted onto synthetic complete (SC) plates containing either fluconazole (FLC) or voriconazole (VRC) at the indicated concentrations. Plates were incubated at 30°C for 48 h. MICs were determined by a broth microdilution method. (B) Ire1 and calcineurin serve redundant roles in cell growth under certain stress conditions in C. glabrata. The assay was performed as in part A. C. glabrata strains: Wild-type, CBS138; Δire1, TG121; Δcnb1, TG161; and Δcnb1 Δire1, TG1612.