MAP-Kinase Regulated Cytosolic Phospholipase A2 Activity Is Essential for Production of Infectious Hepatitis C Virus Particles
Figure 3
Py-2 inhibits production of infectious particles in a virus-specific fashion.
Influence of Py-2 on VSV replication and virus production. (A) Huh7.5 cells were infected with VSV*MQ for 4 h and treated with Py-2 as outlined in Figure 1A. RNA replication and viral protein expression was determined by FACS analysis of GFP expression 24 and 30 h post inoculation. (B) Production of infectious viral progeny at these time points was assessed using a limiting dilution infection assay. The impact of Py-2 treatment on DENV was investigated by inoculation of Huh-7.5 cells with DENV-2 strain 16681 at an MOI of 0.3. The abundance of DENV RNA in the cell lysate (C) and culture fluid (D) at 48 hpi was determined by quantitative RT-PCR (means +/− SD are given). (E) Production of infectious particles was quantified at 48 hpi using a limiting dilution assay. (F) Influence of Py-2 on DENV cell entry was determined by addition of Py-2 to DENV particles prepared in the absence of the drug.