Control of Virulence by Small RNAs in Streptococcus pneumoniae
Figure 3
Involvement of many sRNAs in pathogenesis.
A. Mice were challenged intranasally with the parental TIGR4 strain (filled circles) or the indicated sRNA mutant (open squares). Data represents the overall survival of at least 10 mice from 2 independent experiments. P<0.05 for all the mutants shown by Mantel-Cog log rank test. B. Northern blots of the sRNAs involved in pathogenesis arranged adjacent to the corresponding deletion mutant (top panels). Loading controls against gyrA are shown in the bottom panels. The F22 sRNA was not detectable by Northern, and hence the Ct values for the qRT-PCR for F22 and gyrA in both the TIGR4 and ΔF22 are presented.