Productive Hepatitis C Virus Infection of Stem Cell-Derived Hepatocytes Reveals a Critical Transition to Viral Permissiveness during Differentiation
Figure 5
Cellular determinants of HCV susceptibility.
(A) Induction of microRNA miR-122 expression by FGF-10 during hepatic specification. Equal amounts of total cellular RNA from various cells at the indicated days were subjected to a real-time RT-PCR assay for detection of miR-122 expression. (B) Microarray heat map of gene expression levels in day-10 versus day-7 cells. Two independent RNA samples were processed for each time point. The numbers represent the average values and standard deviations. The conventional color spectrum with green representing downregulation and red representing upregulation was adopted. Fold of changes were also listed next to the name of the gene. (C) Quantitative RT-PCR results of EGFR and EphA2 induction. (D) Upregulation of PI4KIIIα protein during the differentiation process. The levels of CyPA and DDX-3 remained unchanged in the same samples. (E) Quantitative RT-PCR results of IFITM1 and IFI30 expression induction.