In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice
Figure 5
Phenotypical description of B. melitensis infected granulomas in the spleen.
Wild-type C57BL/6 mice were injected i.p. with PBS or 108 CFU of mCherry-Br. Mice were sacrificed at 120 h p.i. and spleens were collected and examined by immunohistofluorescence. A, Immunofluorescence analysis of Ly-6G, CD90.2, CD11b, CD11c, F4/80, and MHC-II expressing cells and mCherry-Br. B, Percentage of iNOS+ cells that colocalize with CD11b-, and CD11c-expressing cells. Numbers in B indicate the percentage of colocalizing cells in the upper panel. Images represent a single granuloma. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.