Inflammatory Monocytes and Neutrophils Are Licensed to Kill during Memory Responses In Vivo
Figure 6
Cytosolic bacteria are engulfed and killed via ROI in the vacuoles of phagocytes during a protective secondary response.
In (A), primary and immune mice (4–6/group) were challenged with 3×105 wt- or ΔActA- L029 Lm and spleens were harvested 24 hrs later and treated as described in figure 5. Histograms show the frequencies (mean +/− SE) of cytosolic (blue bars) and vacuolar (yellow bars) Lm CFUs in phagocytes in a pool of 2 experiments. In (B), primary and memory mice (9–12/group) were treated or not with anti-CD8 or anti-CCL3 before challenge with 3×105 wt-L029 Lm. Histograms indicate as in (A) in a pool of 3 experiments (n = 9). In (C), primary and memory p47phox−/− and C57BL/6 wt control mice (5–6/group) were challenged with 3×105 wt-L029 Lm. Data indicate the number (mean +/− SE) of cytosolic and vacuolar Lm CFUs in phagocytes (n = 5).