Latent KSHV Infection of Endothelial Cells Induces Integrin Beta3 to Activate Angiogenic Phenotypes
Figure 6
Capillary morphogenesis of KSHV-infected cells requires αVβ3 integrin.
A)TIME cells were mock- or KSHV-infected and, 48 hpi, plated on Matrigel in the presence of either RAD- or RGD-containing peptide in complete medium. B) The data in the graphs are the mean number of capillaries per field (left) and the percent of RAD peptide controls (right). C–D) TIME cells were mock- (C) or KSHV-infected (D), and, at 24 hpi, transfected with either control siRNA or siRNA specific to integrin β3. Cells were stained with antibody to mouse IGG (red line) or αVβ3 integrin (blue line: control siRNA; green line: integrin β3 siRNA) and analyzed by flow cytometry. E) At 72 hours post infection and 48 hours post transfection of integrin β3 siRNA, mock- and KSHV-infected cells were plated on Matrigel in complete media and cultured for 6 hours. F) Quantitation of three separate experiments similar to the one shown in E. G) TIME cells were mock- or KSHV-infected and, 48 hpi, plated on Matrigel in the presence of either DMSO, SKI-1, or su6656. H) Quantification of three separate experiments similar to that shown in G. Asterisks indicate a p-value of less than 0.05 compared to DMSO or negative control. Double asterisks indicate a p-value of less than 0.005 compared to DMSO control.