The SARS-Coronavirus-Host Interactome: Identification of Cyclophilins as Target for Pan-Coronavirus Inhibitors
Figure 8
Effect of Cyclosporin A on human (SARS-CoV, HCoV-229E-luc and HCoV-NL63), animal CoV (FCoV, TGEV, IBV) and control virus (HIV-1/EMCV) replication.
SARS-CoV, and EMCV were plaque-titrated on VeroE6, IBV-Beaudette in Vero cells, HCoV-NL63 on CaCo-2, TGEV PUR46 on St-cells, FCoV Black and FCoV 791146 on FCWF cells. HCoV-229E-luc was titrated on Huh-7 Lunet and HIV-1 on C8166 SEAP cells. Data shown are mean values of at least three experiments. HIV-1 data show one representative experiment out of three, values are averages of triplicates. Left and right Y-axes represent the percentage of virus replication and cell viability with the mock-treated cells set as 100%, respectively. CspA concentrations used for each virus are given on the x-axis. The graphs were plotted using the Fit Spline algorithm of Prism Software 4.0 (for Mac) of Graphpad Software Inc. The 50% effective dose (EC50) was calculated by regression analysis of the respective virus CPE.