Suppression of Methylation-Mediated Transcriptional Gene Silencing by βC1-SAHH Protein Interaction during Geminivirus-Betasatellite Infection
Figure 8
A mutation in βC1 blocks both TGS reversal and SAHH interaction.
N. benthamiana plants containing a transcriptionally silenced GFP transgene (line 16-TGS) were inoculated as indicated and photographed under UV light 14 days post-inoculation. (A) Line 16-TGS plants were inoculated with TYLCCNV, or co-inoculated with TYLCCNV + TYLCCNB (expressing wild-type βC1) or TYLCCNB-3A, expressing βC1 in which lysine residues 49-51 are replaced by alanine (βC13A). (B) Line 16-TGS plants were inoculated with the PVX vector, PVX expressing wild-type βC1 (PVX-βC1), or mutant βC1 (PVX-βC13A). (C) BiFC interaction. Constructs expressing βC1, βC13A, and SAHH fused to the N- or C-terminal portions of YFP were delivered to N. benthamiana leaf cells by agroinfiltration. Cells were photographed 48 hours post-infiltration using a confocal laser scanning microscope. The co-expressed proteins are indicated to the left of the photographs.